Transient Activation of GABAB Receptors Suppresses SK Channel Currents in Substantia Nigra Pars Compacta Dopaminergic Neurons
| Autor: | D. James Surmeier, Paul Greengard, Joshua A. Goldberg, Lars Brichta, Chad M Estep, Daniel J. Galtieri, Enrico Zampese |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Patch-Clamp Techniques Potassium Channels Small-Conductance Calcium-Activated Potassium Channels Physiology lcsh:Medicine Action Potentials Stimulation Biochemistry Ion Channels Adenylyl Cyclase Signaling Cascade Intracellular Receptors Mice 0302 clinical medicine Cell Signaling Postsynaptic potential Animal Cells Medicine and Health Sciences GABAergic Neurons lcsh:Science gamma-Aminobutyric Acid Neurons Multidisciplinary Chemistry GABAA receptor Physics Neurochemistry Neurotransmitters Signaling Cascades Electrophysiology Physical Sciences GABAergic Female Cellular Types Neurochemicals Ion Channel Gating Adenylyl Cyclases Research Article Signal Transduction Signal Inhibition Biophysics Neurophysiology Substantia nigra GABAB receptor Membrane Potential SK channel 03 medical and health sciences Animals Pars Compacta Pars compacta Dopaminergic Neurons lcsh:R Biology and Life Sciences Proteins Cell Biology Receptors GABA-A Cyclic AMP-Dependent Protein Kinases Mice Inbred C57BL 030104 developmental biology nervous system Receptors GABA-B Cellular Neuroscience lcsh:Q Neuroscience Dopaminergics 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 12, p e0169044 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) are richly innervated by GABAergic neurons. The postsynaptic effects of GABA on SNc DA neurons are mediated by a mixture of GABAA and GABAB receptors. Although activation of GABAA receptors inhibits spike generation, the consequences of GABAB receptor activation are less well characterized. To help fill this gap, perforated patch recordings were made from young adult mouse SNc DA neurons. Sustained stimulation of GABAB receptors hyperpolarized SNc DA neurons, as previously described. However, transient stimulation of GABAB receptors by optical uncaging of GABA did not; rather, it reduced the opening of small-conductance, calcium-activated K+ (SK) channels and increased the irregularity of spiking. This modulation was attributable to inhibition of adenylyl cyclase and protein kinase A. Thus, because suppression of SK channel activity increases the probability of burst spiking, transient co-activation of GABAA and GABAB receptors could promote a pause-burst pattern of spiking. |
| Databáze: | OpenAIRE |
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