Bendamustine, but not fludarabine, exhibits a low stem cell toxicity in vitro
| Autor: | Antonia Busse, Martin Schmidt-Hieber, Birgit Reufi, W. U. Knauf, I. W. Blau, Eckhard Thiel |
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| Rok vydání: | 2008 |
| Předmět: |
Bendamustine
Cancer Research Cell Survival Cell Culture Techniques Antineoplastic Agents Bone Marrow Cells Models Biological Colony-Forming Units Assay Reference Values Granulocyte Colony-Stimulating Factor medicine Bendamustine Hydrochloride Humans Progenitor cell Cytotoxicity Clonogenic assay Chemistry Stem Cells General Medicine Hematopoietic Stem Cells Fludarabine Haematopoiesis Oncology Apoptosis Nitrogen Mustard Compounds Cancer research Blood Component Removal Stem cell Vidarabine medicine.drug |
| Zdroj: | Journal of cancer research and clinical oncology. 135(2) |
| ISSN: | 1432-1335 |
| Popis: | We investigated the in vitro toxicity of bendamustine and fludarabine to hematopoietic progenitors and stem cells from healthy donors.Clonogenic agar colony assays, non-clonogenic long-term liquid cultures (LTC) and apoptosis assays were used to assess the cytotoxicity of both the agents.Total colony-forming units (CFU) were more sensitive to fludarabine than to bendamustine in agar colony assays (IC(50) 0.7 microM/L and 8.5 microM/L, respectively). Using the Bliss independence model and combining the two agents yielded additive inhibition of progenitors. Non-clonogenic assays, including LTC and an apoptosis assay detecting activated caspases showed that stem cells are characterized by low sensitivity to bendamustine. In contrast, fludarabine strongly inhibited the viability and growth of stem cells in LTC.Our data show that bendamustine is characterized by lower in vitro toxicity to hematopoietic progenitors and stem cells than fludarabine and might thus be preferable in regimens prior to stem cells apheresis. |
| Databáze: | OpenAIRE |
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