Synthesis and Evaluation of In Vitro DNA/Protein Binding Affinity, Antimicrobial, Antioxidant and Antitumor Activity of Mononuclear Ru(II) Mixed Polypyridyl Complexes

Autor: Venkat Reddy Putta, Nagamani Chintakuntla, Rajender Reddy Mallepally, Srishailam Avudoddi, Nagasuryaprasad K., Deepika Nancherla, Yaswanth V. V. N, Prakasham R. S., Satyanarayana Singh Surya, Satyanarayana Sirasani
Rok vydání: 2015
Předmět:
Sociology and Political Science
Stereochemistry
Phenanthroline
Clinical Biochemistry
Molecular Conformation
chemistry.chemical_element
Antineoplastic Agents
Microbial Sensitivity Tests
010402 general chemistry
Gram-Positive Bacteria
01 natural sciences
Biochemistry
Antioxidants
Ruthenium
HeLa
chemistry.chemical_compound
Bipyridine
Structure-Activity Relationship
Gram-Negative Bacteria
Organometallic Compounds
Animals
Humans
MTT assay
Spectroscopy
Bacillus megaterium
Cell Proliferation
Fluorescent Dyes
Binding Sites
biology
Dose-Response Relationship
Drug
010405 organic chemistry
Acridine orange
Serum Albumin
Bovine
Nuclear magnetic resonance spectroscopy
Cell Cycle Checkpoints
DNA
biology.organism_classification
0104 chemical sciences
Anti-Bacterial Agents
Clinical Psychology
chemistry
Cattle
Drug Screening Assays
Antitumor
Law
Social Sciences (miscellaneous)
HeLa Cells
Phenanthrolines
Zdroj: Journal of fluorescence. 26(1)
ISSN: 1573-4994
Popis: The four novel Ru(II) complexes [Ru(phen)2MAFIP](2+) (1) [MAFIP = 2-(5-(methylacetate)furan-2-yl)-1 H-imidazo[4,5-f] [1, 10]phenanthroline, phen = 1,10-Phenanthroline], [Ru(bpy)2MAFIP](2+) (2) (bpy = 2,2'-bipyridine) and [Ru(dmb)2MAFIP](2+) (3) (dmb = 4,4'-dimethyl-2,2'-bipyridine) and [Ru(hdpa)2MAFIP](2+) (4) (hdpa = 2,2-dipyridylamine) have been synthesized and fully characterized via elemental analysis, NMR spectroscopy, EI-MS and FT-IR spectroscopy. In addition, the DNA-binding behaviors of the complexes 1-4 with calf thymus DNA were investigated by UV-Vis absorption, fluorescence studies and viscosity measurement. The DNA-binding experiments showed that the complexes 1-4 interact with CT-DNA through an intercalative mode. BSA protein binding affinity of synthesized complexes was determined by UV/Vis absorption and fluorescence emission titrations. The binding affinity of ruthenium complexes was supported by molecular docking. The photoactivated cleavage of plasmid pBR322 DNA by ruthenium complexes 1-4 was investigated. All the synthesized compounds were tested for antimicrobial activity by using three Gram-negative (Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa) and three Gram-positive (Micrococcus luteus, Bacillus subtilis and Bacillus megaterium) organisms, these results indicated that complex 3 was more activity compared to other complexes against all tested microbial strains while moderate antimicrobial activity profile was noticed for complex 4. The antioxidant activity experiments show that the complexes exhibit moderate antioxidant activity. The cytotoxicity of synthesized complexes on HeLa cell lines has been examined by MTT assay. The apoptosis assay was carried out with Acridine Orange (AO) staining methods and the results indicate that complexes can induce the apoptosis of HeLa cells. The cell cycle arrest investigated by flow cytometry and these results indicate that complexes 1-4 induce the cell cycle arrest at G0/G1 phase.
Databáze: OpenAIRE
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