Interlaboratory Reproducibility of Selective Reaction Monitoring Assays Using Multiple Upfront Analyte Enrichment Strategies
| Autor: | Alessandra Luchini, Andrei P. Drabovich, Vathany Kulasingam, Bryan Krastins, Randall W. Nelson, Ihor Batruch, Daniel W. Chan, Paul E. Oran, Mary F. Lopez, Yuan Tian, Amol Prakash, David A. Sarracino, Claudia Fredolini, Christopher R. Smith, Taha Rezai, Yan Li, Paul Russo, Hui Zhang, Emanuel F. Petricoin, Michael Athanas, Eleftherios P. Diamandis, Lance A. Liotta |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Analyte Interlaboratory reproducibility Selective reaction Operating procedures Molecular Sequence Data Analytical chemistry Mass spectrometry Biochemistry Mass Spectrometry Article Limit of Detection Humans Multiplex Amino Acid Sequence Detection limit Chromatography Reverse-Phase Reproducibility Chromatography Human Growth Hormone Chemistry Seminal Plasma Proteins Prostatic Neoplasms Reproducibility of Results General Chemistry Prostate-Specific Antigen Reference Standards Peptide Fragments Female Laboratories Blood Chemical Analysis |
| Zdroj: | Journal of Proteome Research. 11:3986-3995 |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | Over the past few years, mass spectrometry has emerged as a technology to complement and potentially replace standard immunoassays in routine clinical core laboratories. Application of mass spectrometry to protein and peptide measurement can provide advantages including high sensitivity, the ability to multiplex analytes, and high specificity at the amino acid sequence level. In our previous study, we demonstrated excellent reproducibility of mass spectrometry-selective reaction monitoring (MS-SRM) assays when applying standardized standard operating procedures (SOPs) to measure synthetic peptides in a complex sample, as lack of reproducibility has been a frequent criticism leveled at the use of mass spectrometers in the clinical laboratory compared to immunoassays. Furthermore, an important caveat of SRM-based assays for proteins is that many low-abundance analytes require some type of enrichment before detection with MS. This adds a level of complexity to the procedure and the potential for irreproducibility increases, especially across different laboratories with different operators. The purpose of this study was to test the interlaboratory reproducibility of SRM assays with various upfront enrichment strategies and different types of clinical samples (representing real-world body fluids commonly encountered in routine clinical laboratories). Three different, previously published enrichment strategies for low-abundance analytes and a no-enrichment strategy for high-abundance analytes were tested across four different laboratories using different liquid chromatography-SRM (LC-SRM) platforms and previously developed SOPs. The results demonstrated that these assays were indeed reproducible with coefficients of variation of less than 30% for the measurement of important clinical proteins across all four laboratories in real world samples. |
| Databáze: | OpenAIRE |
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