Characterization of a monoclonal antibody against a 24KD antigen from rat testis important for fertility regulation
| Autor: | Chandrima Shaha, T. Sheshadri, Anil Suri, Gursaran P. Talwar |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
endocrine system medicine.drug_class Immunology Clone (cell biology) Hamster Biology Cross Reactions In Vitro Techniques Monoclonal antibody Antigen Cricetinae Testis Genetics medicine Animals Humans Antigens Acrosome Sperm-Ovum Interactions urogenital system Antibodies Monoclonal Rats Inbred Strains Oocyte Sperm Agglutination Molecular biology Sperm Rats Molecular Weight medicine.anatomical_structure Fertility biology.protein Female Antibody |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0272-457X |
| Popis: | Monoclonal antibodies were raised against a 24KD antigen from rat testicular cytosol which was previously shown to produce antibody titres in rats with inhibitory effect on fertility. Of the 20 hybridoma clones selected for study, one clone HS-D5 was finally chosen for characterisation as it proved to be functionally promising. The clone was secreting IgM type of antibody. it produced strong agglutination of human sperm and prevented binding of hamster sperm to hamster oocyte. In localization studies it identified the acrosome of sperm of multiple species. Western blots with rat testicular cytosol and human sperm extracts showed a strong band at around 24KD with HS-D5. On Western blots from two dimensional gels, HS-D5 identified multiple spots at the region of 24KD. The mechanism of action of the antibody seemed to be at the level of interaction of the oocyte and spermatozoa. |
| Databáze: | OpenAIRE |
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