Lophirones B and C induce oxidative cellular death pathway in Acinetobacter baumannii by inhibiting DNA gyrase
| Autor: | N O Aliyu, T.O. Ajiboye, R.A. Ajala-Lawal |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Acinetobacter baumannii Iron 030106 microbiology Mutant Oxidative phosphorylation Microbial Sensitivity Tests medicine.disease_cause Microbiology DNA gyrase 03 medical and health sciences Minimum inhibitory concentration chemistry.chemical_compound Chalcones Superoxides polycyclic compounds medicine Topoisomerase II Inhibitors Microbial Viability biology Superoxide Hydrogen Peroxide biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Molecular biology Anti-Bacterial Agents Oxidative Stress 030104 developmental biology Infectious Diseases chemistry DNA Gyrase bacteria NAD+ kinase Oxidative stress |
| Zdroj: | Microbial pathogenesis. 130 |
| ISSN: | 1096-1208 |
| Popis: | We evaluated the inactivation of DNA gyrase on the oxidative stress response and sensitivity of A. baumannii to lophirones B and C. The sensitivity of parental and the mutant strains of A. baumannii to lophirones B and C was determined using minimum inhibitory concentration (MIC) and time-kill sensitivity. Inactivation of sodB, katG, recA enhanced the sensitivity of A. baumannii to lophirones B and C. Furthermore, this inactivation increased the accumulation of superoxide anion radical and hydrogen peroxide in lophirones B and C-treated A. baumannii, which was reversed in the presence of thiourea. Inactivation of gyrA stalled lophirones B and C-mediated ROS accumulation in A. baumannii. In addition, lophirones B and C raised the Fe2+ contents of A. baumannii. Dipyridyl (Fe chelator) reversed the sensitivity of A. baumannii to lophirones B and C. Lophirones significantly lowered the NAD+/NADH ratio of A. baumannii. The results of this study revealed that the impact of DNA gyrase in lophirones B and C-mediated ROS accumulation, Fe2+ release and cell death. |
| Databáze: | OpenAIRE |
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