Cloning, characterization, and expression of a cDNA encoding an inducible nitric oxide synthase from the human chondrocyte
| Autor: | Mark S. Hickery, Ian G. Charles, Richard M.J. Palmer, Salvador Moncada, Ann Chubb, David W. Moss, Michael T. Bayliss, Valerie S. Hall |
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| Rok vydání: | 1993 |
| Předmět: |
DNA
Complementary Molecular Sequence Data CHO Cells Molecular cloning Transfection Chondrocyte Cricetinae Complementary DNA Gene expression medicine Animals Humans Amino Acid Sequence Cloning Molecular Peptide sequence chemistry.chemical_classification Multidisciplinary Base Sequence Sequence Homology Amino Acid biology ATP synthase Molecular biology Recombinant Proteins Amino acid Enzyme Activation Nitric oxide synthase Cartilage medicine.anatomical_structure Genes Oligodeoxyribonucleotides chemistry Biochemistry biology.protein Amino Acid Oxidoreductases Nitric Oxide Synthase Sequence Alignment Research Article Interleukin-1 |
| Popis: | Incubation of human articular chondrocytes with interleukin 1 beta results in the time-dependent expression of nitric oxide (NO) synthase. We report here the isolation of a cDNA clone which encodes a protein of 1153 amino acids with a molecular mass of 131,213 Da and a calculated isoelectric point of 7.9. CHO cells transfected with a plasmid harboring this cDNA clone expressed NO synthase activity that was inhibited by some L-arginine analogues. The deduced amino acid sequence of the human chondrocyte inducible NO synthase shows 51% identity and 68% similarity with the endothelial NO synthase and 54% identity and 70% similarity with the neuronal NO synthase. The similarity (88%) between the human chondrocyte NO synthase cDNA sequence and that reported for the murine macrophage suggests that the inducible class of enzyme is conserved between different cell types and across species. |
| Databáze: | OpenAIRE |
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