Potential of Silver Nanoparticles in Overcoming the Intrinsic Resistance of Pseudomonas aeruginosa to Secondary Metabolites from Carnivorous Plants
| Autor: | Zbigniew Kaczyński, Magdalena Narajczyk, Joanna E. Frackowiak, Aleksandra Królicka, Natalia Grzegorczyk, Marta Krychowiak-Maśnicka, Aleksandra Bielicka-Giełdoń, Mirosława Krauze-Baranowska, Sylwia Godlewska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Drosera spp QH301-705.5 030106 microbiology synergy naphthoquinone medicine.disease_cause Catalysis Silver nanoparticle Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound medicine Physical and Theoretical Chemistry Biology (General) Molecular Biology fractional bactericidal concentration QD1-999 Spectroscopy activity modulation Pseudomonas aeruginosa Organic Chemistry Broth microdilution Pathogenic bacteria General Medicine Plumbagin Antimicrobial Naphthoquinone antagonism Computer Science Applications Chemistry 030104 developmental biology chemistry Biochemistry Phytochemical Dionaea muscipula |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 4849, p 4849 (2021) International Journal of Molecular Sciences Volume 22 Issue 9 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Carnivorous plants are exemplary natural sources of secondary metabolites with biological activity. However, the therapeutic antimicrobial potential of these compounds is limited due to intrinsic resistance of selected bacterial pathogens, among which Pseudomonas aeruginosa represents an extreme example. The objective of the study was to overcome the intrinsic resistance of P. aeruginosa by combining silver nanoparticles (AgNPs) with secondary metabolites from selected carnivorous plant species. We employed the broth microdilution method, the checkerboard titration technique and comprehensive phytochemical analyses to define interactions between nanoparticles and active compounds from carnivorous plants. It has been confirmed that P. aeruginosa is resistant to a broad range of secondary metabolites from carnivorous plants, i.e., naphthoquinones, flavonoids, phenolic acids (MBC = 512 µg mL−1) and only weakly sensitive to their mixtures, i.e., extracts and extracts’ fractions. However, it was shown that the antimicrobial activity of extracts and fractions with a significant level of naphthoquinone (plumbagin) was significantly enhanced by AgNPs. Our studies clearly demonstrated a crucial role of naphthoquinones in AgNPs and extract interaction, as well as depicted the potential of AgNPs to restore the bactericidal activity of naphthoquinones towards P. aeruginosa. Our findings indicate the significant potential of nanoparticles to modulate the activity of selected secondary metabolites and revisit their antimicrobial potential towards human pathogenic bacteria. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|