Doxorubicin delivery by magnetic nanotheranostics enhances the cell death in chemoresistant colorectal cancer-derived cells
| Autor: | Pamela Liliana Azcona, Verónica Leticia Lassalle, María Julia Martín, Claudia Gentili |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Programmed cell death
Colorectal cancer Poly ADP ribose polymerase Pharmaceutical Science Context (language use) 02 engineering and technology 030226 pharmacology & pharmacy Theranostic Nanomedicine 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Humans Doxorubicin Magnetite Nanoparticles Cell Death Chemistry Magnetic Phenomena 021001 nanoscience & nanotechnology medicine.disease Apoptosis Drug delivery Cancer research Magnetic nanoparticles 0210 nano-technology Colorectal Neoplasms medicine.drug |
| Zdroj: | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 158 |
| ISSN: | 1879-0720 |
| Popis: | Colorectal cancer (CRC) is a major cause of cancer death with a high probability of treatment failure. Doxorubicin (DOXO) is an efficient antitumor drug; however, most CRC cells show resistance to its effects. Magnetic nanoparticles (MNPs) are potential cancer management tools that can serve as diagnostic agents and also can optimize and personalize treatments. This work aims to evaluate the aptitude of magnetic nanotheranostics composed of magnetite (Fe3O4) nanoparticles coated with folic acid intended to the sustained release of DOXO. The administration of DOXO by means of these MNPs resulted in the enhancement of cell death respect to the free drug administration. Chromatin compaction and cytoplasmic protrusions were observed. Mitochondrial transmembrane potential disruption and increased PARP protein cleavage confirmed apoptosis. The nanosystem was also tested as a vectoring tool by exposing it to the stimuli of a static magnetic field in vitro. CRC-related magnetic nanotechnology still remains in pre-clinical trials. In this context, this contribution expands the knowledge of the behavior of MNPs in contact with in vitro models and proposes the nanodevices studied here as potential theranostic agents for the monitoring of the progress of CRC and the evolution of its treatment. |
| Databáze: | OpenAIRE |
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