Preparation of injectable 3D-formed .BETA.-tricalcium phosphate bead/alginate composite for bone tissue engineering
| Autor: | Yasuhiko Tabata, Tazuko Satoh, Yoshikazu Umezu, Masaaki Nakamura, Tomonori Matsuno, Yoshiya Hashimoto, Yasuyuki Ozeki, Kazuhiko Omata, Seita Adachi, Yamauchi Yoshitaka |
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| Rok vydání: | 2008 |
| Předmět: |
Calcium Phosphates
Scaffold Bone Regeneration Materials science Compressive Strength Alginates Surface Properties Scanning electron microscope Dermatologic Surgical Procedures Osteocalcin Composite number Cell Culture Techniques Mice Nude Biocompatible Materials Bead Injections Calcium Chloride Mice chemistry.chemical_compound Tissue engineering Osteogenesis Absorbable Implants Materials Testing Animals Humans General Dentistry Alginic acid Aqueous solution Tissue Engineering Tissue Scaffolds Guided Tissue Regeneration Cell Differentiation Mesenchymal Stem Cells Alkaline Phosphatase Cross-Linking Reagents Compressive strength chemistry visual_art Microscopy Electron Scanning Ceramics and Composites visual_art.visual_art_medium Stress Mechanical Biomarkers Biomedical engineering |
| Zdroj: | Dental Materials Journal. 27:827-834 |
| ISSN: | 1881-1361 0287-4547 |
| DOI: | 10.4012/dmj.27.827 |
| Popis: | A novel, injectable bone tissue engineering material was developed that consisted of beta-tricalcium phosphate (beta-TCP) beads as the solid phase and alginate as the gel phase. To prepare the instantaneously formed composite scaffold, an aqueous calcium chloride solution was dried on the surface of beta-TCP beads and crosslinked with an alginic acid sodium solution, thereby forming stable beta-TCP beads and alginate gel which were injectable via a syringe. This biodegradable composite was a three-dimensional (3D) material that could be used as an injectable scaffold for bone tissue engineering. In particular, the composite with 2.0 wt% alginate concentration exhibited a compressive strength of 69 kPa in dry conditions, which was significantly higher than that exhibited by 1.0 wt%. Furthermore, mesenchymal stem cells (MSC) were 3D-cultured within the composite and then investigated for osteogenic markers. MSC-loaded composite was subjected to scanning electron microscope (SEM) examination and implanted subcutaneously for in vivo experiment. Results showed that the scaffold provided support for osteogenic differentiation. In light of the encouraging results obtained, this novel injectable composite material may be useful for bone tissue engineering. |
| Databáze: | OpenAIRE |
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