Effectiveness of purified methylene blue in an experimental model of Mycobacterium ulcerans infection
| Autor: | Shady Asmar, Roger B. D. Tian, Michel Drancourt, Claude Napez, Hubert Lepidi |
|---|---|
| Přispěvatelé: | Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes (URMITE), INSB-INSB-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48 |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Buruli ulcer Male medicine.medical_treatment 030106 microbiology Intraperitoneal injection Microbial Sensitivity Tests Microbiology 03 medical and health sciences chemistry.chemical_compound [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases medicine Animals Humans Pharmacology (medical) Buruli Ulcer Mycobacterium marinum Mice Inbred BALB C biology Standard treatment Nontuberculous Mycobacteria General Medicine biology.organism_classification medicine.disease Bacterial Load 3. Good health Anti-Bacterial Agents Methylene Blue Disease Models Animal Infectious Diseases Treatment Outcome chemistry Streptomycin Mycobacterium ulcerans Female Methylene blue Rifampicin medicine.drug |
| Zdroj: | International Journal of Antimicrobial Agents International Journal of Antimicrobial Agents, Elsevier, 2017, 49 (3), pp.290-295. ⟨10.1016/j.ijantimicag.2016.11.012⟩ International Journal of Antimicrobial Agents, 2017, 49 (3), pp.290-295. ⟨10.1016/j.ijantimicag.2016.11.012⟩ |
| ISSN: | 1872-7913 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2016.11.012⟩ |
| Popis: | International audience; Mycobacterium ulcerans is responsible for Buruli ulcer, characterised by extensive, disabling ulcers. Standard treatment combining rifampicin and streptomycin exposes patients to toxicity and daily painful injections. In this study, the in vitro susceptibilities of 3 M. ulcerans strains, 1 Mycobacterium marinum strain and 18 strains representative of eleven other Mycobacterium species and subspecies to methylene blue were determined. Whilst growth of M. ulcerans was inhibited by 0.0125 g/L methylene blue, growth of all other tested strains was not inhibited by 1 g/L methylene blue. The effectiveness of methylene blue in a murine model of M. ulcerans infection was then tested. Topical treatment by brushing a methylene blue solution on the skin lesion, systemic treatment by intraperitoneal injection of methylene blue, and a combined treatment (topical and systemic) were tested. The three treatment groups exhibited a significantly lower clinical score compared with the non-treated control group (P |
| Databáze: | OpenAIRE |
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