Transporter genes identified in landraces associated with high zinc in polished rice through panicle transcriptome for biofortification

Autor: K. Surekha, C. N. Neeraja, V. Ravindra Babu, Kalyani S. Kulkarni, D. Sanjeeva Rao, P. Madhu Babu
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Biofortification
lcsh:Medicine
Gene Expression
Plant Science
Biochemistry
01 natural sciences
Transcriptome
Inbred strain
Medicine and Health Sciences
lcsh:Science
education.field_of_study
Multidisciplinary
Plant Anatomy
Nutritional Deficiencies
Eukaryota
food and beverages
Genomics
Plants
Marker-assisted selection
Micronutrient
Up-Regulation
Chemistry
Zinc
Horticulture
Experimental Organism Systems
Micronutrient Deficiencies
Physical Sciences
Transcriptome Analysis
Research Article
Chemical Elements
Inflorescences
Population
Down-Regulation
chemistry.chemical_element
Biology
Genes
Plant
Real-Time Polymerase Chain Reaction
Research and Analysis Methods
03 medical and health sciences
Plant and Algal Models
DNA-binding proteins
Genetics
Gene Regulation
Grasses
Panicles
education
Nutrition
Panicle
lcsh:R
Organisms
Membrane Transport Proteins
Correction
Biology and Life Sciences
Proteins
Computational Biology
Oryza
Genome Analysis
Regulatory Proteins
030104 developmental biology
chemistry
lcsh:Q
Rice
Microsatellite Repeats
Transcription Factors
010606 plant biology & botany
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 2, p e0192362 (2018)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0192362
Popis: Polished rice is poor source of micronutrients, however wide genotypic variability exists for zinc uptake and remobilization and zinc content in brown and polished grains in rice. Two landraces (Chittimutyalu and Kala Jeera Joha) and one popular improved variety (BPT 5204) were grown under zinc sufficient soil and their analyses showed high zinc in straw of improved variety, but high zinc in polished rice in landraces suggesting better translocation ability of zinc into the grain in landraces. Transcriptome analyses of the panicle tissue showed 41182 novel transcripts across three samples. Out of 1011 differentially expressed exclusive transcripts by two landraces, 311 were up regulated and 534 were down regulated. Phosphate transporter-exporter (PHO), proton-coupled peptide transporters (POT) and vacuolar iron transporter (VIT) showed enhanced and significant differential expression in landraces. Out of 24 genes subjected to quantitative real time analyses for confirmation, eight genes showed significant differential expression in landraces. Through mapping, six rice microsatellite markers spanning the genomic regions of six differentially expressed genes were validated for their association with zinc in brown and polished rice using recombinant inbred lines (RIL) of BPT 5204/Chittimutyalu. Thus, this study reports repertoire of genes associated with high zinc in polished rice and a proof concept for deployment of transcriptome information for validation in mapping population and its use in marker assisted selection for biofortification of rice with zinc.
Databáze: OpenAIRE
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