Chromosome‐scale genome assembly and insights into the metabolome and gene regulation of leaf color transition in an important oak species, Quercus dentata

Autor: Wen‐Bo Wang, Xiang‐Feng He, Xue‐Mei Yan, Bo Ma, Cun‐Fu Lu, Jing Wu, Yi Zheng, Wen‐He Wang, Wen‐Bo Xue, Xue-Chan Tian, Jing-Fang Guo, Yousry A. El‐Kassaby, Ilga Porth, Ping‐Sheng Leng, Zeng‐Hui Hu, Jian‐Feng Mao
Rok vydání: 2023
Předmět:
Zdroj: New Phytologist. 238:2016-2032
ISSN: 1469-8137
0028-646X
DOI: 10.1111/nph.18814
Popis: Quercus dentata Thunb., a dominant forest tree species in northern China, has significant ecological and ornamental value due to its adaptability and beautiful autumn coloration, with color changes from green to yellow into red resulting from the autumnal shifts in leaf pigmentation. However, the key genes and molecular regulatory mechanisms for leaf color transition remain to be investigated. First, we presented a high-quality chromosome-scale assembly for Q. dentata. This 893.54 Mb sized genome (contig N50 = 4.21 Mb, scaffold N50 = 75.55 Mb; 2n = 24) harbors 31 584 protein-coding genes. Second, our metabolome analyses uncovered pelargonidin-3-O-glucoside, cyanidin-3-O-arabinoside, and cyanidin-3-O-glucoside as the main pigments involved in leaf color transition. Third, gene co-expression further identified the MYB-bHLH-WD40 (MBW) transcription activation complex as central to anthocyanin biosynthesis regulation. Notably, transcription factor (TF) QdNAC (QD08G038820) was highly co-expressed with this MBW complex and may regulate anthocyanin accumulation and chlorophyll degradation during leaf senescence through direct interaction with another TF, QdMYB (QD01G020890), as revealed by our further protein–protein and DNA–protein interaction assays. Our high-quality genome assembly, metabolome, and transcriptome resources further enrich Quercus genomics and will facilitate upcoming exploration of ornamental values and environmental adaptability in this important genus.
Databáze: OpenAIRE
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