Relationship of phospholipid chemistry to serological reactivity in the Venereal Disease Research Laboratory slide test antigen
| Autor: | L Pine, M W Reeves, B McLaurin, B E McGrew |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Microbiology (medical)
Cardiolipins Chemistry Phospholipid chemistry.chemical_element Flocculation Tests Peroxides Syphilis Serodiagnosis Serology chemistry.chemical_compound Biochemistry Antigen BEEF HEART Fatty Acids Unsaturated Phosphatidylcholines lipids (amino acids peptides and proteins) Fatty acid composition Antigens Cobalt Phospholipids Research Article Flocculation test |
| Zdroj: | Journal of Clinical Microbiology. 14:48-54 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | A total of 13 egg lecithins, 12 beef heart lecithins, and 15 beef heart cardiolipins were assayed for the ability to function in the Venereal Disease Research Laboratory microflocculation test, as well as for purity, fatty acid composition, free amines, metals, and products of oxidation. We found that the presence of peroxides and oxidation-related ultraviolet-absorbing chromophores showed a close inverse relationship to acceptable serological activity. The degree of purity of the lipids had only a slight influence on serological activity, whereas fatty acid composition, saturation, and configuration had none at all. We did not detect contaminating iron, copper, cobalt, nickel, or free amines in these lipids. We discuss the implications of our findings for improving the chemical standards for these lipids. |
| Databáze: | OpenAIRE |
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