Efficient Induction of Apoptosis by Wee1 Kinase Inhibition in Hepatocellular Carcinoma Cells
| Autor: | Shunichi Ariizumi, Etsuko Hashimoto, Tomomi Kogiso, Keiko Shiratori, Hikaru Nagahara, Masakazu Yamamoto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Carcinoma
Hepatocellular Cellular differentiation lcsh:Medicine Gene Expression Apoptosis Cell Cycle Proteins Gastroenterology and Hepatology Transforming Growth Factor beta1 Cyclin-dependent kinase Cell Line Tumor CDC2 Protein Kinase Medicine and Health Sciences Humans lcsh:Science Protein Kinase Inhibitors Cyclin-dependent kinase 1 Multidisciplinary biology Kinase Cell growth lcsh:R Liver Neoplasms Biology and Life Sciences Nuclear Proteins Cell Biology Protein-Tyrosine Kinases Protein kinase R Molecular biology Immunohistochemistry digestive system diseases Cyclin-Dependent Kinases Enzyme Activation Wee1 Oncology biology.protein Cancer research lcsh:Q RNA Interference Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 6, p e100495 (2014) |
| ISSN: | 1932-6203 |
| Popis: | Transforming growth factor-β1 (TGF-β1) potently inhibits human hepatocellular carcinoma (HCC) cell growth. Here we demonstrated that TGF-β1-induced apoptosis is mediated by decreased phosphorylation of cdc2 at Tyr15 accompanied by down-regulation of Wee1 kinase expression. As expected from these results, a Wee1 kinase inhibitor efficiently induced apoptosis in HCC cells in the absence of TGF-β1 treatment. In surgically resected samples, Wee1 kinase was expressed in moderately to poorly differentiated HCC, whereas no Wee1 kinase expression was observed in non-cancerous tissue, including cirrhotic tissue. Our results suggest that Wee1 kinase inhibitors may be a practical novel therapeutic option against advanced HCC. |
| Databáze: | OpenAIRE |
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