The Fab region of IgG2 human myeloma proteins does not bear the streptococcal protein G-specific determinant
| Autor: | G Luccarelli, M Neri, Franco Dammacco, Federico Perosa |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Myeloma protein
Immunology Chromatography Affinity Subclass Epitopes Immunoglobulin Fab Fragments Bacterial Proteins Antibody Specificity Streptococcal protein G Humans Immunology and Allergy Bovine serum albumin Incubation Immunosorbent Techniques Antigens Bacterial biology Chemistry Antibodies Monoclonal Streptococcus Igg subclasses Molecular biology Myeloma Proteins Biochemistry Immunoglobulin G biology.protein Protein G Myeloma igg |
| Zdroj: | Journal of Immunological Methods. 203:153-155 |
| ISSN: | 0022-1759 |
| Popis: | Seven out of ten Fab (F(ab')2/Fab') preparations derived from purified human myeloma IgG showed a substantial binding to protein G-Sepharose. Subclass analysis revealed that the 7 protein G-reactive Fabs included 3 IgG1, 2 IgG3 and 2 IgG4 Fabs, whereas the remaining 3 which were not adsorbed were IgG2 Fab. Incubation of protein G-Sepharose with non-saturating amounts of 4 Fab preparations, representative of all IgG subclasses, showed that gamma 1, gamma 3 and gamma 4 Fabs adsorbed from 26 to 28.3%, whereas 80% of gamma 2 Fab was left in the supernatant after adsorption. These results indicate that human IgG2 lack PG-specific Fab-associated reactive site(s). |
| Databáze: | OpenAIRE |
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