Expression of A-kinase anchor protein 13 and Rho-associated coiled-coil containing protein kinase in restituted and regenerated mucosal epithelial cells following mucosal injury and colorectal cancer cells in mouse models
| Autor: | Naomi Koyama, Akira Kataoka, Shim-mo Hayashi, Makoto Shibutani, Toshinori Yoshida, Tomoka Ohsumi, Yumi Kangawa, Takeshi Tanaka |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty medicine.medical_treatment Azoxymethane A Kinase Anchor Proteins Biology Toxicology medicine.disease_cause Pathology and Forensic Medicine Minor Histocompatibility Antigens 03 medical and health sciences Mice medicine Animals Guanine Nucleotide Exchange Factors Regeneration ROCK1 ROCK2 Colitis Intestinal Mucosa Protein kinase A Lamina propria Mice Inbred BALB C Wound Healing rho-Associated Kinases Dextran Sulfate Cell Biology General Medicine medicine.disease Molecular biology Epithelium Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cytokine Female Carcinogenesis Colorectal Neoplasms |
| Zdroj: | Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. 69(7) |
| ISSN: | 1618-1433 |
| Popis: | We demonstrate the expression patterns of A-kinase anchor protein 13 (AKAP13), a scaffold protein that acts upstream of Rho signaling, and Rho-associated coiled-coil containing protein kinase (ROCK) 1/2 in mouse colorectal cancer and during the healing stage of mouse colitis. BALB/c mice received an intraperitoneal injection of azoxymethane at 10mg/kg, followed by two 7-day cycles of 3% dextran sulfate sodium (DSS) administered through their drinking water to induce colon cancer, or a 7-day administration of 4% DSS to induce colitis. The colorectal tissue was then analyzed for gene expression, histopathology, and immunohistochemistry. In the colorectal cancer, AKAP13 and ROCK1/2 were highly expressed in adenocarcinoma compared to the control tissue and low-grade dysplasia. In colitis, AKAP13 and ROCK1 were highly expressed in the restituted and regenerated mucosa but were only moderately expressed in the injured mucosal epithelium, compared to the normal epithelium that exhibited weak expression levels. ROCK2 was weakly expressed in these cells, consistent with the expression of AKAP13 and ROCK1. Furthermore, we found several clumps of epithelial cells expressing AKAP13 and ROCK1/2 in the lamina propria during the mucosal healing process, and these cells also expressed interleukin-6, which is a multipotential cytokine for both inflammation and healing. These data suggest that AKAP13 was expressed in relation with ROCK1/2, which probably play an overall role in both mucosal healing and tumorigenesis. |
| Databáze: | OpenAIRE |
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