Identification of cell-surface markers for detecting breast cancer cells in ovarian tissue
| Autor: | Alexander L. Vahrmeijer, Inge T. A. Peters, Cornelis F. M. Sier, Carina G. J. M. Hilders, J. Baptist Trimbos, Peter J. K. Kuppen, Vincent T.H.B.M. Smit |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Fluorescence-lifetime imaging microscopy Pathology medicine.medical_specialty Receptor ErbB-2 medicine.medical_treatment Breast Neoplasms Receptors Urokinase Plasminogen Activator 03 medical and health sciences 0302 clinical medicine Breast cancer Antigens CD Obstetrics and Gynaecology Autotransplantation Biomarkers Tumor Humans Medicine Ovarian tissue cryopreservation Fertility preservation Cryopreservation Ovarian Neoplasms 030219 obstetrics & reproductive medicine Cluster of differentiation business.industry Ovarian tissue Ovary Obstetrics and Gynecology General Medicine Middle Aged Gynecologic Oncology Cadherins medicine.disease Immunohistochemistry 3. Good health 030220 oncology & carcinogenesis Tumor markers Female Breast cancer cells business |
| Zdroj: | Archives of Gynecology and Obstetrics Archives of Gynecology and Obstetrics, 294(2), 385-393 |
| Popis: | Purpose The safety of ovarian tissue autotransplantation in oncology patients cannot be ensured, as current tumor-detection methods compromise the ovarian tissue viability. Although non-destructive methods (for instance near-infrared fluorescence imaging) can discriminate malignant from healthy tissues while leaving the examined tissues unaffected, they require specific cell-surface tumor markers. We determined which tumor markers are suitable targets for tumor-specific imaging to exclude the presence of breast cancer cells in ovarian tissue. Methods Immunohistochemistry was performed on formalin-fixed, paraffin-embedded specimens of ten ovaries from premenopausal patients. Additionally, we screened a tissue microarray containing tumor tissue cores from 24 breast cancer patients being eligible for ovarian tissue cryopreservation. The following cell-surface tumor markers were tested: E-cadherin, EMA (epithelial membrane antigen), Her2/neu (human epidermal growth factor receptor type 2), αvβ6 integrin, EpCAM (epithelial cell adhesion molecule), CEA (carcinoembryonic antigen), FR-α (folate receptor-alpha), and uPAR (urokinase-type plasminogen activator receptor). For each tumor, the percentage of positive breast tumor cells was measured. Results None of the ten ovaries were positive for any of the markers tested. However, all markers (except CEA and uPAR) were present on epithelial cells of inclusion cysts. E-cadherin was present in the majority of breast tumors: ≥90 % of tumor cells were positive for E-cadherin in 17 out of 24 tumors, and 100 % of tumor cells were positive in 5 out of 24 tumors. Conclusions Of the markers tested, E-cadherin is the most suitable marker for a tumor-specific probe in ovarian tissue. Methods are required to distinguish inclusion cysts from breast tumor cells. |
| Databáze: | OpenAIRE |
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