Local-Level Genetic Diversity and Structure of Matsutake Mushroom (Tricholoma matsutake) Populations in Nagano Prefecture, Japan, Revealed by 15 Microsatellite Markers
| Autor: | Shuichi Asakawa, Yoshie Takeuchi, Engkong Tan, Shijie Zhang, Hiroyuki Kurokochi, Chunlan Lian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Microbiology (medical) Population Zoology Plant Science 010603 evolutionary biology 01 natural sciences Analysis of molecular variance Article Fixation index 03 medical and health sciences genetic diversity private allele microsatellite markers genet genetic conservation education lcsh:QH301-705.5 Ecology Evolution Behavior and Systematics Isolation by distance Genetics education.field_of_study Genetic diversity biology Matsutake biology.organism_classification Transplantation 030104 developmental biology lcsh:Biology (General) Genetic structure |
| Zdroj: | Journal of Fungi; Volume 3; Issue 2; Pages: 23 Journal of Fungi Journal of Fungi, Vol 3, Iss 2, p 23 (2017) |
| ISSN: | 2309-608X |
| DOI: | 10.3390/jof3020023 |
| Popis: | The annual yield of matsutake mushrooms (Tricholoma matsutake) has consistently decreased in Japan over the past few decades. We used 15 polymorphic and codominant simple sequence repeat (SSR) markers, developed using next-generation sequencing, to carry out genetic analyses of 10 populations in Nagano, Japan. Using the SSRs, we identified 223 genotypes, none of which was observed in more than one population. The mean expected heterozygosity and standardized allelic richness values were 0.67 and 4.05, respectively. Many alleles appeared in only one of the 10 populations; 34 of these private alleles were detected with a mean number per population of 3.4. The fixation index (FST) and standardized genetic differentiation (G′ST) values were 0.019 and 0.028, respectively. Analysis of molecular variance (AMOVA) showed that the contribution of among population, among genets within a population, and within genets variation to the total variation was 2.91%, 11.62%, and 85.47%, respectively, with genetic differentiation being detected for all sources. Twenty-eight of 45 pairwise FST values were significantly larger than zero, and no pattern of isolation by distance was detected among the 10 populations. Bayesian-based clustering did not show clear differences among populations. These results suggest that reestablishment of a colony would be best accomplished by transplantation within a field; if this is not possible, then transplantation from within several dozen kilometers will cause little damage to the original population genetic structure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|