Erythrocyte glutathione deficiency in symptom-free HIV infection is associated with decreased synthesis rate
| Autor: | William C. Heird, Alan Jackson, Brian Gazzard, Margaret Frazer, Danny Sharpstone, Farook Jahoor, Gary Philips |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Erythrocytes Physiology Endocrinology Diabetes and Metabolism Glycine HIV Infections Biology Asymptomatic Virus chemistry.chemical_compound Acquired immunodeficiency syndrome (AIDS) Reference Values Physiology (medical) Immunopathology Extracellular medicine Humans Osmolar Concentration Glutathione biology.organism_classification medicine.disease Acetylcysteine Kinetics chemistry Lentivirus Immunology Viral disease medicine.symptom |
| Zdroj: | The American journal of physiology. 276(1) |
| ISSN: | 0002-9513 |
| Popis: | Although several studies have documented intra- and extracellular glutathione (GSH) deficiency in asymptomatic human immunodeficiency virus (HIV) infection, the mechanisms responsible for the altered GSH homeostasis remain unknown. To determine whether decreased synthesis contributes to this alteration of GSH homeostasis, a primed-constant infusion of [2H2]glycine was used to measure the fractional and absolute rates of synthesis of GSH in five healthy and five symptom-free HIV-infected subjects before and after supplementation for 1 wk with N-acetylcysteine. The erythrocyte GSH concentration of the HIV-infected group was lower ( P < 0.01) than that of the control group (1.4 ± 0.16 vs. 2.4 ± 0.08 mmol/l). The smaller erythrocyte GSH pool of the HIV-infected group was associated with a significantly slower ( P < 0.01) absolute synthesis rate of GSH (1.15 ± 0.14 vs. 1.71 ± 0.15 mmol ⋅ l−1 ⋅ day−1) compared with controls. Cysteine supplementation elicited significant increases in both the absolute rate of synthesis and the concentration of erythrocyte GSH. These results suggest that the GSH deficiency of HIV infection is due in part to a reduced synthesis rate secondary to a shortage in cysteine availability. |
| Databáze: | OpenAIRE |
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