Wnt Signaling Drives Prostate Cancer Bone Metastatic Tropism and Invasion
Autor: | Jae Seung Chung, Hao Zhang, Yugang Wang, Frank C. Cackowski, Therese M. Roth, Ganesh S. Palapattu, Kenneth J. Pienta, Russell S. Taichman, Alexander Zaslavsky, Farah Chammaa, Huiru Zhao, Yuanyuan Qiao, Tadas Kasputis, Jacob A. Belardo, Todd M. Morgan, Arul M. Chinnaiyan, Udit Singhal |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
musculoskeletal diseases
0301 basic medicine Cancer Research Original article animal structures Stromal cell Biology WIF1 lcsh:RC254-282 Metastasis 03 medical and health sciences Prostate cancer 0302 clinical medicine medicine Femur Wnt signaling pathway Osteoblast musculoskeletal system medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Bone marrow |
Zdroj: | Translational Oncology, Vol 13, Iss 4, Pp-(2020) Translational Oncology |
ISSN: | 1936-5233 |
Popis: | Wnt signaling has been implicated as a driver of prostate cancer-related osteoblast differentiation, and previous studies have linked modifications in Wnt function with the induction of tumor metastasis. A unique aspect of prostate cancer bone metastases in mouse models is their relative predilection to the hindlimb (femur) compared to the forelimb (humerus). Comparative gene expression profiling was performed within the humerus and femur from non–tumor-bearing mice to evaluate differences in the microenvironments of these locations. This revealed the relative overexpression of the Wnt signaling inhibitors WIF1 and SOST in the humerus compared to the femur, with increased WNT5A expression in femur bone marrow, suggesting a coordinated upregulation of Wnt signals within the femur compared to the humerus. Conditioned medium (CM) from bone marrow stromal cells (HS-5 cells) was used to mimic the bone marrow microenvironment, which strongly promoted prostate cancer cell invasion (3.3-fold increase in PC3 cells, P |
Databáze: | OpenAIRE |
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