Investigation of treatment-time differences in colistin-induced nephrotoxicity in Wistar rats
| Autor: | Temidayo Olutoyin Olurishe, A B Olorukooba, Joshua E Eronmosele |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Physiology 030209 endocrinology & metabolism Pharmacology Kidney medicine.disease_cause Nephrotoxicity 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) Animals Humans Medicine Circadian rhythm Rats Wistar Creatinine Colistin business.industry Malondialdehyde Anti-Bacterial Agents Circadian Rhythm Rats chemistry Toxicity Urea business 030217 neurology & neurosurgery Oxidative stress medicine.drug |
| Zdroj: | Chronobiology International. 38:224-233 |
| ISSN: | 1525-6073 0742-0528 |
| DOI: | 10.1080/07420528.2020.1838535 |
| Popis: | Colistin-induced nephrotoxicity (CIN) occurs in up to 60% of patients, and this has restricted its clinical use. In view of its efficacy amidst the rising challenge of infections caused by multidrug-resistant bacteria, current studies are focusing on ways to ameliorate colistin-induced nephrotoxicity. This study investigated treatment-time differences in colistin-induced nephrotoxicity in Wistar rats. A dose of 600,000 IU/Kg/day of colistimethate sodium (CMS) was administered to male Wistar rats to induce nephrotoxicity; the rats tolerated the higher dose for the treatment duration with higher mean values of serum creatinine, urea, and malondialdehyde compared to the group that received 450,000 IU/Kg/day CMS (p ≤ 0.05). Four groups (n = 8/group) of rats received intraperitoneal (i.p.) injections of 600,000 IU/Kg/day CMS each at four equally spaced circadian times (00:00, 06:00, 12:00, and 18:00 h) to determine the time of administration with least renal toxicity. Biomarkers of oxidative stress and renal toxicity were measured and kidney histology studied after the treatments. The results showed a 24-h pattern in nephrotoxicity from CIN, and that treatment during the activity time period (dark phase) caused lowest CIN. Histological findings supported this finding, with photomicrographs consistently showing more pronounced features of CIN in the groups treated during time frame that coincided with the rest phase in rats (12:00 and 18:00). |
| Databáze: | OpenAIRE |
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