Evolutionarily conserved function of the sacred lotus (Nelumbo nucifera Gaertn.) CER2-LIKE family in very-long-chain fatty acid elongation
| Autor: | Matthew A. Jenks, Longyu Huang, Huayan Zhao, Baiming Yang, Xianpeng Yang, Zhouya Wang, Tao Feng, Shiyou Lü, Juanjuan Li, Pingfang Yang |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fatty Acid Elongases Very long chain fatty acid Lotus Arabidopsis Plant Science Fatty acid elongase complex Nelumbo Genes Plant Real-Time Polymerase Chain Reaction 01 natural sciences Evolution Molecular 03 medical and health sciences chemistry.chemical_compound Acetyltransferases Botany Genetics Arabidopsis thaliana Gene family Conserved Sequence Phylogeny Plant Proteins biology Reverse Transcriptase Polymerase Chain Reaction Fatty Acids Plants Genetically Modified biology.organism_classification Plant Leaves 030104 developmental biology chemistry Fatty acid elongation Petal 010606 plant biology & botany |
| Zdroj: | Planta. 248:715-727 |
| ISSN: | 1432-2048 0032-0935 |
| DOI: | 10.1007/s00425-018-2934-6 |
| Popis: | Identification of NnCER2 and NnCER2-LIKE from Nelumbo nucifera, which are required for the very-long-chain fatty acid elongation, provides new evidence that CER2 proteins are evolutionarily conserved across the eudicots. CER2-LIKE family proteins have been described as core components of the fatty acid elongase complex in Arabidopsis, maize, and rice, having specific function in synthesis of the C30 to C34 fatty acyl-CoA precursors of cuticular waxes. Little is known about the functional conservation in this gene family across species. In this study, two CER2-LIKE family proteins, NnCER2 and NnCER2-LIKE, were characterized from sacred lotus (Nelumbo nucifera), which is an ancient basal eudicot. The transcriptional expression of NnCER2 and NnCER2-LIKE was found in floating leaf blades, emergent petioles and vertical leaves, petals, and anthers. The NnCER2 and NnCER2-LIKE proteins were localized to the endoplasmic reticulum and nucleus. Overexpressing NnCER2 and NnCER2-LIKE in Arabidopsis led to alteration of cuticle wax structure in inflorescence stems, and this was associated with elevated 30, 32, and 34 carbon length wax compounds, and their derivatives. The different substrate specificities of NnCER2 and NnCER2-LIKE were explored using co-expression with AtCER6 in yeast cells. These findings provide clear evidence that the function of CER2 family proteins in producing VLCFAs is highly conserved across the eudicots. |
| Databáze: | OpenAIRE |
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