ANTIBACTERIAL AND ANTIVIRAL ACTIVITIES OF STINGING NETTLE (URTICA DIOICA L.) LEAF EXTRACT ON NOROVIRUS AND CAMPYLOBACTER JEJUNI AS FOODBORNE PATHOGENS
| Autor: | Hanife Banu Aydın, Serol Korkmaz, Burcu Irem Omurtag Korkmaz |
|---|---|
| Přispěvatelé: | Aydin H. B., Korkmaz S., OMURTAG KORKMAZ B. İ. |
| Rok vydání: | 2023 |
| Předmět: |
Tarımsal Bilimler
Agricultural Sciences Life Sciences norovirus Agriculture campylobacter Agriculture & Environment Sciences (AGE) Yemek bilimi Microbiology food safety Ziraat Food Engineering GIDA BİLİMİ VE TEKNOLOJİSİ Yaşam Bilimleri Tarım Bilimleri Engineering and Technology cytotoxicity Mühendislik ve Teknoloji Tarım ve Çevre Bilimleri (AGE) Molecular Biology Gıda Mühendisliği urtica dioica L FOOD SCIENCE & TECHNOLOGY Food Science Biotechnology |
| Zdroj: | Journal of microbiology, biotechnology and food sciences. :e9768 |
| ISSN: | 1338-5178 |
| DOI: | 10.55251/jmbfs.9768 |
| Popis: | Research background. The cytotoxicity on various vital cell lines and the activity on foodborne pathogens (murine norovirus 1 as a norovirus surrogate and Campylobacter jejuni) of methanolic Urtica dioica L. leaf extract (UDE) were studied. Experimental approach. The cytotoxic concentration of 50% (CC50) was measured by the linearity between UDE concentrations and cell viability. Antibacterial effects on C. jejuni were analyzed by the broth microdilution method with a spectrophotometer. The virucidal and antiviral activities of UDE were determined by the virus titration method on the host cell infectivity and expressed as the tissue cell infective dose of 50% using the method of Spearman–Karber. Results and conclusions. The CC50 of UDE was determined on macrophage as the virus host cell. MIC and MBC of UDE were determined as 5 mg/ml and 20 mg/ml for both C. jejuni isolated from poultry meat and the standard strain. UDE inhibited MNV-1 on three pathways of host cell infectivity at approximately the same 50% inhibitory concentration (1.45-1.87 mg/ml). In conclusion, the present study tried to explain in detail the dose-dependent activity of Urtica dioica L. leaf extract on two important foodborne pathogens causing outbreaks worldwide. The results showed that it might be a safe and alternative food additive and supplement candidate at safe concentrations. |
| Databáze: | OpenAIRE |
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