Enhanced collateral growth by double transplantation of gene-nucleofected fibroblasts in ischemic hindlimb of rats
| Autor: | Wulf D. Ito, Daniel Müller, Hans-Günther Machens, Natalie Lund, Shaoxiang Weng, Astrid Arnold, Jessica Nehlsen, Ziyang Zhang, Alex Slobodianski, Jihong Liu, Jörn A. Lohmeyer, José-Tomás Egaña |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Angiogenesis Valvular Disease Genetic enhancement Ischemia Collateral Circulation Neovascularization Physiologic lcsh:Medicine Hindlimb Biology Cardiovascular Neovascularization Andrology Vascular Biology medicine Animals lcsh:Science Cells Cultured Burn Management Multidisciplinary lcsh:R Fibroblasts Reconstructive Surgery Collateral circulation medicine.disease Rats Surgery Transplantation Medicine Fibroblast Growth Factor 2 lcsh:Q medicine.symptom Ex vivo Plasmids Research Article |
| Zdroj: | Plos One Artículos CONICYT CONICYT Chile instacron:CONICYT PLoS ONE, Vol 6, Iss 4, p e19192 (2011) PLoS ONE |
| Popis: | Background Induction of neovascularization by releasing therapeutic growth factors is a promising application of cell-based gene therapy to treat ischemia-related problems. In the present study, we have developed a new strategy based on nucleofection with alternative solution and cuvette to promote collateral growth and re-establishment of circulation in ischemic limbs using double transplantation of gene nucleofected primary cultures of fibroblasts, which were isolated from rat receiving such therapy. Methods and Results Rat dermal fibroblasts were nucleofected ex vivo to release bFGF or VEGF165 in a hindlimb ischemia model in vivo. After femoral artery ligation, gene-modified cells were injected intramuscularly. One week post injection, local confined plasmid expression and transient distributions of the plasmids in other organs were detected by quantitative PCR. Quantitative micro-CT analyses showed improvements of vascularization in the ischemic zone (No. of collateral vessels via micro CT: 6.8±2.3 vs. 10.1±2.6; p |
| Databáze: | OpenAIRE |
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