Dose-dependent effect of continuous subcutaneous verapamil infusion on experimental acute pancreatitis in mice
| Autor: | G. Lake-Bakaar, S. Lyubsky |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Normal diet Physiology Trypsinogen Cytoplasmic Granules Choline Mice chemistry.chemical_compound Methionine Internal medicine Zymogen medicine Zymogen granule membrane Animals Ethionine Pancreas Enzyme Precursors Dose-Response Relationship Drug business.industry Gastroenterology Infusion Pumps Implantable Calcium Channel Blockers Zymogen granule medicine.disease Endocrinology Pancreatitis Verapamil chemistry Acute Disease Acute pancreatitis Female business medicine.drug |
| Zdroj: | Digestive Diseases and Sciences. 40:2349-2355 |
| ISSN: | 1573-2568 0163-2116 |
| DOI: | 10.1007/bf02063236 |
| Popis: | Calcium antagonists may limit experimental tissue injury by membrane stabilization. We studied the effects of verapamil on pancreatic ultrastructure and zymogen extraction during diet-induced acute pancreatitis. Acute pancreatitis was induced in female Swiss-Webster mice by feeding a choline- and methionine-deficient diet (CD) supplemented with 1% ethionine (CDE). Varying doses of verapamil in normal saline were infused continuously at a rate of 0.5μl/hr for 96 hr through subcutaneously implanted osmotic pumps. The pancreata were examined blindly by light microscopy and by electron microscopy. Zymogen extracted from pancreatic tissue was measured and expressed per gram of protein. Mean histological scores, calculated according to a formula that incorporates the extent of necrosis, inflammation, acidophilia, and edema, were 14.1±4, 10.3±2, 9.9±4, 5.9±7, 12.5±4, and 12.7±4 for CDE-fed animals receiving 0, 0.14, 0.28, 0.56, 0.84, and 1.12μM verapamil daily, respectively. Animals fed normal diet or CD had scores of 0±0. Histological scores were significantly lower in animals treated with 0.56μm verapamil compared to animals who received no verapamil (p |
| Databáze: | OpenAIRE |
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