Prelytic stimulation of target and effector cells following conjugation as measured by intracellular fluorescein fluorescence polarization
| Autor: | Shlomo Lalchuk, Oleg Marder, Reuven Tirosh, Dror Fixler, Avi Eisenthal, Mordechai Deutsch, Yosef Irlin |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
education.field_of_study
Effector Lymphocyte Population Biomedical Engineering Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Biomaterials Cytosol chemistry.chemical_compound medicine.anatomical_structure chemistry Immunology medicine Biophysics sense organs Fluorescein education Fluorescence anisotropy Intracellular K562 cells |
| Zdroj: | Journal of biomedical optics. 3(3) |
| ISSN: | 1083-3668 |
| Popis: | The aim of the present study was to detect prelytic intracellular changes induced in target and effector cells following their conjugation at room temperature. Changes in the cytoplasmic matrix were measured by means of intracellular fluorescein fluorescence polarization (IFFP) using the Cellscan apparatus. Both natural killer and lymphocyte activated killer cells were used as effector cells, while K562 and Daudi cell lines were used as targets. The results show that following their conjugation, both the effector and the target cells show significant reductions (gt;10%) in IFFP values. Changes in IFFP were induced by specific interaction and only between viable cells. No evidence of fluorescein transfer from a stained cell to its nonstained counterpart was found. To the best of our knowledge, this is the first time that effector-target interaction is monitored on an individual cell basis within a population, by means of IFFP measurements. In addition, in order to explain the physical phenomena, measurements of physical parameters which might affect the IFFP, such as changes in osmolality and pH, were performed and discussed. © 1998 Society of Photo-Optical Instrumentation Engineers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|