Bioinformatic Analysis of Plasma Apolipoproteins A-I and A-II Revealed Unique Features of A-I/A-II HDL Particles in Human Plasma
| Autor: | Mitsuyo Okazaki, Toshimi Kido, Hiroshige Itakura, Kazuo Kondo, Shinji Yokoyama, Takeyoshi Urata, Hideaki Kurata |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Genotype Apolipoprotein A-II 030204 cardiovascular system & hematology Apolipoproteins A Lipid Metabolism Inborn Errors Article 03 medical and health sciences 0302 clinical medicine Molar ratio Internal medicine Cholesterylester transfer protein medicine polycyclic compounds Humans Immunoelectrophoresis Multidisciplinary biology Apolipoprotein A-I Chemistry Wild type nutritional and metabolic diseases Computational Biology Heterozygote advantage Cholesterol Ester Transfer Proteins 030104 developmental biology Endocrinology Human plasma biology.protein lipids (amino acids peptides and proteins) Female Lipoproteins HDL |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep31532 |
| Popis: | Plasma concentration of apoA-I, apoA-II and apoA-II-unassociated apoA-I was analyzed in 314 Japanese subjects (177 males and 137 females), including one (male) homozygote and 37 (20 males and 17 females) heterozygotes of genetic CETP deficiency. ApoA-I unassociated with apoA-II markedly and linearly increased with HDL-cholesterol, while apoA-II increased only very slightly and the ratio of apoA-II-associated apoA-I to apoA-II stayed constant at 2 in molar ratio throughout the increase of HDL-cholesterol, among the wild type and heterozygous CETP deficiency. Thus, overall HDL concentration almost exclusively depends on HDL with apoA-I without apoA-II (LpAI) while concentration of HDL containing apoA-I and apoA-II (LpAI:AII) is constant having a fixed molar ratio of 2 : 1 regardless of total HDL and apoA-I concentration. Distribution of apoA-I between LpAI and LpAI:AII is consistent with a model of statistical partitioning regardless of sex and CETP genotype. The analysis also indicated that LpA-I accommodates on average 4 apoA-I molecules and has a clearance rate indistinguishable from LpAI:AII. Independent evidence indicated LpAI:A-II has a diameter 20% smaller than LpAI, consistent with a model having two apoA-I and one apoA-II. The functional contribution of these particles is to be investigated. |
| Databáze: | OpenAIRE |
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