The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia
| Autor: | Stefan Monecke, Maciej Korus, Andrea T. Feßler, Adriana Cabal, Ferenc Kiskaroly, Tanja Bernreiter-Hofer, Werner Ruppitsch, Elke Müller, Igor Loncaric, Franz Allerberger, Dusan Misic, Michael P. Szostak, Stefan Schwarz, Damir Benković, Viktoria Milovanovic, Ralf Ehricht, Joachim Spergser, Sascha D. Braun, Małgorzata Korzeniowska |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
RM1-950 Biology medicine.disease_cause 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten E. coli Biochemistry Microbiology biocides resistance Plasmid mcr medicine turkeys Pharmacology (medical) Typing Replicon colistin General Pharmacology Toxicology and Pharmaceutics Escherichia coli Phylogenetic tree OmpT Infectious Diseases Colistin MCR-1 Therapeutics. Pharmacology medicine.drug 600 Technik Medizin angewandte Wissenschaften::630 Landwirtschaft::636 Viehwirtschaft |
| Zdroj: | Antibiotics, Vol 10, Iss 1063, p 1063 (2021) Antibiotics Volume 10 Issue 9 |
| Popis: | The aim of this study was continuous monitoring of the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry at intensive breeding facilities in Northern Vojvodina, Serbia, from 1 January 1 to 1 October 2020. Out of 2167 examined samples, mcr-1 was observed in five E. coli isolates originating from healthy turkeys. Four isolates belonged to the phylogenetic group B1, and one isolate to the phylogenetic group A. Detected E. coli serogenotypes (somatic O and flagellar H antigens) were O8:H25 and O29:H25. Core-genome multi-locus sequence typing (cgMLST) revealed three ST58 isolates clustering together in Clonal Complex (CC) 155 and two singletons of ST641-CC86 and ST410-CC23, respectively. Clonotyping revealed CH4-32 (n = 3), CH6-53 (n = 1) and CH4-24 (n = 1). In all isolates, the mcr-1 gene was located on a large IncX4 replicon type plasmid. Eight virulence-associated genes (VAGs) typical of avian pathogenic E. coli (APEC) (fyuA, fimH, hlyF, iss, ompT, sitA, traT, iroN) were detected in four isolates. These isolates were investigated for susceptibility to four biocides and revealed MIC values of 0.125% for glutardialdehyde, of 0.00003–0.00006% for chlorohexidine, of 4–6% for isopropanol and of 0.001–0.002% for benzalkonium chloride. All obtained MIC values of the tested biocides were comparable to the reference strain, with no indication of possible resistance. This is the first report of mcr-1.1-carrying E. coli from Serbia. Although only samples from turkeys were mcr-positive in this study, continuous monitoring of livestock samples is advised to prevent a spill-over from animals to humans. |
| Databáze: | OpenAIRE |
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