Effect of tunicamycin on maturation of fetal mouse lung
| Autor: | S. R. Hilfer, Robert L. Searls, J. Kornilow, E. H. Webster |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Pulmonary and Respiratory Medicine
Glycosylation Time Factors Physiology Mesenchyme Mice Inbred Strains Biology Extracellular matrix Embryonic and Fetal Development Mice chemistry.chemical_compound Physiology (medical) medicine Animals Tissue Distribution Type-I Pneumocytes Respiratory system Lung Tunicamycin Type-II Pneumocytes Proteins Cell Differentiation Cell Biology Embryo Mammalian Cell biology medicine.anatomical_structure chemistry Immunology Autoradiography Basal lamina Mannose |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 265:L250-L259 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.1993.265.3.l250 |
| Popis: | The mesodermal capsule of the fetal lung plays a role in differentiation of the respiratory region. It has been proposed for other epithelial organs that the mesodermal capsule influences development by modifying the basal lamina or the extended extracellular matrix. The effect could be on deposition or turnover of collagens, proteoglycans, and/or glycoproteins. This study tests the role of glycoproteins in differentiation of respiratory endings by inhibiting their synthesis with the antibiotic tunicamycin (TM). Lungs at 16 and 18 days gestation and 3 days after birth were cultured with TM and examined for morphological and biochemical differences from normal controls. With TM, alveolar regions did not expand properly and formed fewer type I pneumocytes, although type II pneumocytes were unaffected. The epithelium of untreated respiratory regions showed greater incorporation of radioactive mannose than the airways region or mesenchyme. This incorporation was diminished in TM, but the pattern persisted. Comparison with the results obtained with beta-xyloside suggested that differentiation of type I and type II pneumocytes is under separate control. |
| Databáze: | OpenAIRE |
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