Sarcoma Spheroids and Organoids-Promising Tools in the Era of Personalized Medicine

Autor: Flavio Fazioli, Carlo Ruosi, Michele Gallo, Gaetano Apice, Annarosaria De Chiara, Amelia Cimmino, Gianluca Colella, Filomena de Nigris
Přispěvatelé: Colella, Gianluca, Fazioli, Flavio, Gallo, Michele, De Chiara, Annarosaria, Apice, Gaetano, Ruosi, Carlo, Cimmino, Amelia, De Nigris, Filomena
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Disease
Review
Catalysi
lcsh:Chemistry
0302 clinical medicine
Tumor Cells
Cultured

Tumor Microenvironment
sarcomas
lcsh:QH301-705.5
Spectroscopy
Computer Science Applications1707 Computer Vision and Pattern Recognition
Sarcoma
General Medicine
personalized medicine
3. Good health
Computer Science Applications
030220 oncology & carcinogenesis
Human
precision medicine
spheroids
Computational biology
Biology
Models
Biological

Catalysis
tumor microenvironment
Inorganic Chemistry
03 medical and health sciences
Spheroids
Cellular

medicine
Animals
Humans
Epigenetics
Physical and Theoretical Chemistry
Molecular Biology
Tumor microenvironment
business.industry
Animal
Mesenchymal stem cell
Organic Chemistry
medicine.disease
Precision medicine
030104 developmental biology
Clinical research
Spheroid
lcsh:Biology (General)
lcsh:QD1-999
Personalized medicine
business
Zdroj: International journal of molecular sciences
19 (2018): 615. doi:10.3390/ijms19020615
info:cnr-pdr/source/autori:Colella, Gianluca; Fazioli, Flavio; Gallo, Michele; De Chiara, Annarosaria; Apice, Gaetano; Ruosi, Carlo; Cimmino, Amelia; de Nigris, Filomena/titolo:Sarcoma Spheroids and Organoids-Promising Tools in the Era of Personalized Medicine./doi:10.3390%2Fijms19020615/rivista:International journal of molecular sciences (Print)/anno:2018/pagina_da:615/pagina_a:/intervallo_pagine:615/volume:19
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 19, Iss 2, p 615 (2018)
DOI: 10.3390/ijms19020615
Popis: Cancer treatment is rapidly evolving toward personalized medicine, which takes into account the individual molecular and genetic variability of tumors. Sophisticated new in vitro disease models, such as three-dimensional cell cultures, may provide a tool for genetic, epigenetic, biomedical, and pharmacological research, and help determine the most promising individual treatment. Sarcomas, malignant neoplasms originating from mesenchymal cells, may have a multitude of genomic aberrations that give rise to more than 70 different histopathological subtypes. Their low incidence and high level of histopathological heterogeneity have greatly limited progress in their treatment, and trials of clinical sarcoma are less frequent than trials of other carcinomas. The main advantage of 3D cultures from tumor cells or biopsy is that they provide patient-specific models of solid tumors, and they overcome some limitations of traditional 2D monolayer cultures by reflecting cell heterogeneity, native histologic architectures, and cell– extracellular matrix interactions. Recent advances promise that these models can help bridge the gap between preclinical and clinical research by providing a relevant in vitro model of human cancer useful for drug testing and studying metastatic and dormancy mechanisms. However, additional improvements of 3D models are expected in the future, specifically the inclusion of tumor vasculature and the immune system, to enhance their full ability to capture the biological features of native tumors in high-throughput screening. Here, we summarize recent advances and future perspectives of spheroid and organoid in vitro models of rare sarcomas that can be used to investigate individual molecular biology and predict clinical responses. We also highlight how spheroid and organoid culture models could facilitate the personalization of sarcoma treatment, provide specific clinical scenarios, and discuss the relative strengths and limitations of these models.
Databáze: OpenAIRE