Assembly of TALE-based DNA scaffold for the enhancement of exogenous multi-enzymatic pathway
| Autor: | Si-si Xie, Xin-yuan Qiu, Lv-yun Zhu, Chuanyang Liu, Dong-Yi Zhang, Lingyun Zhu, Chu-shu Zhu, Xiaomin Wu |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Scaffold Mevalonic Acid Bioengineering medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Transcription (biology) 010608 biotechnology Escherichia coli medicine Transcription Activator-Like Effectors chemistry.chemical_classification Effector Robustness (evolution) DNA General Medicine Cell biology Metabolic pathway 030104 developmental biology Enzyme Metabolic Engineering chemistry Metabolic Networks and Pathways Plasmids Biotechnology |
| Zdroj: | Journal of Biotechnology. 296:69-74 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2019.03.008 |
| Popis: | Synthetic scaffold systems, which exhibit enzyme clustering effect, have been considered as an important parallel approach for metabolic flux control and pathway enhancement. Here, we described an improved DNA-based scaffold system for synthetic tri-enzymatic pathway in Escherichia coli. With plasmid DNA serving as scaffold and exogenous enzymes fused with rationally designed transcription activator-like effectors (TALEs), our approach successfully clustered three TALE-fused enzymes and significantly increased the production of a mevalonate-producing tri-enzymatic pathway with the optimized scaffold structure and plasmid copy number. These results further suggested the scalability and robustness of the TALE-based scaffold system, and we can assume that it can be used on numerous multi-enzyme metabolic pathways due to its programmable features. |
| Databáze: | OpenAIRE |
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