The development of a non-invasive behavioral model of thermal heat stress in laboratory mice (Mus musculus)
| Autor: | Nancy J. Flood, Chris Miller, Mark Paetkau, Justin Terrance Mufford, John S. Church, Gilly Regev-Shoshani |
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| Rok vydání: | 2016 |
| Předmět: |
Hot Temperature
Light Infrared Rays Video Recording Motor Activity Biology Heat Stress Disorders Body Temperature Stress (mechanics) 03 medical and health sciences 0302 clinical medicine Telemetry Animals 0501 psychology and cognitive sciences 050102 behavioral science & comparative psychology Simulation Behavior Animal Pigmentation business.industry Air General Neuroscience 05 social sciences Non invasive Thermoregulation Actigraphy Behavioral modeling Mice Inbred C57BL Disease Models Animal Thermography Female business Software 030217 neurology & neurosurgery Thermal energy Body Temperature Regulation Biomedical engineering Vasomotor tone |
| Zdroj: | Journal of Neuroscience Methods. 268:189-195 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/j.jneumeth.2015.12.011 |
| Popis: | Background Many behavioral and physiological studies of laboratory mice employ invasive methods such as radio telemetry to measure key aspects of behavior and physiology. Radio telemetry requires surgical implants, which may impact mouse health and behavior, and thus reduce the reliability of the data collected. New method We developed a method to measure key aspects of thermoregulatory behavior without compromising animal welfare. We examined the thermoregulatory response to heat stress in a custom-built arena that permitted the use of simultaneous and continuous infrared thermography (IRT) and video capture. This allowed us to measure changes in surface body temperature and determine total distance traveled using EthoVision XT animal tracking software. Results Locomotor activity and surface body temperature differed between heat-stressed mice and mice kept within their thermal comfort zone. The former had an increase in surface body temperature and a decline in locomotor activity, whereas the latter had a stable surface body temperature and showed greater activity levels. Methods Surface body temperature and locomotor activity are conventionally quantified by measurements taken at regular intervals, which limit the use and accuracy of the data. We obtained data of high resolution (i.e., recorded continuously) and accuracy that allowed for the examination of key physiological measurements such as energy expenditure and peripheral vasomotor tone. Conclusions This novel experimental method for studying thermoregulatory behavior in mice using non-invasive tools has advantages over radio-telemetry and represents an improvement in laboratory animal welfare. |
| Databáze: | OpenAIRE |
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