Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia
| Autor: | Vanessa L. White, Siu F. Lee, Juli Rochmijati Wuliandari, Warsito Tantowijoyo, Nancy M Endersby-Harshman, Ary A. Hoffmann |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
mosquito
Aedes aegypti Biology Vssc Article High Resolution Melt chemistry.chemical_compound parasitic diseases kdr Allele lcsh:Science Genotyping Genetics Pyrethroid pyrethroid Point mutation Knockdown resistance biology.organism_classification para 3. Good health Deltamethrin chemistry bioassay Insect Science lcsh:Q HRM |
| Zdroj: | Insects, Vol 6, Iss 3, Pp 658-685 (2015) Insects Volume 6 Issue 3 Pages 658-685 |
| ISSN: | 2075-4450 |
| Popis: | Mutations in the voltage-sensitive sodium channel gene (Vssc) have been identified in Aedes aegypti and some have been associated with pyrethroid insecticide resistance. Whether these mutations cause resistance, alone or in combination with other alleles, remains unclear, but must be understood if mutations are to become markers for resistance monitoring. We describe High Resolution Melt (HRM) genotyping assays for assessing mutations found in Ae. aegypti in Indonesia (F1565C, V1023G, S996P) and use them to test for associations with pyrethroid resistance in mosquitoes from Yogyakarta, a city where insecticide use is widespread. Such knowledge is important because Yogyakarta is a target area for releases of Wolbachia-infected mosquitoes with virus-blocking traits for dengue suppression. We identify three alleles across Yogyakarta putatively linked to resistance in previous research. By comparing resistant and susceptible mosquitoes from bioassays, we show that the 1023G allele is associated with resistance to type I and type II pyrethroids. In contrast, F1565C homozygotes were rare and there was only a weak association between individuals heterozygous for the mutation and resistance to a type I pyrethroid. As the heterozygote is expected to be incompletely recessive, it is likely that this association was due to a different resistance mechanism being present. A resistance advantage conferred to V1023G homozygotes through addition of the S996P allele in the homozygous form was suggested for the Type II pyrethroid, deltamethrin. Screening of V1023G and S996P should assist resistance monitoring in Ae. aegypti from Yogyakarta, and these mutations should be maintained in Wolbachia strains destined for release in this city to ensure that these virus-blocking strains of mosquitoes are not disadvantaged, relative to resident populations. |
| Databáze: | OpenAIRE |
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