Effects of artepillin C on model membranes displaying liquid immiscibility

Autor: Wallance Moreira Pazin, Neus Vilanova, Ademilson Espencer Egea Soares, Amando Siuiti Ito, Ilja K. Voets
Přispěvatelé: Universidade de São Paulo (USP), Universidade do Estado de São Paulo, Eindhoven University of Technology, Dutch Polymer Institute (DPI), Institute for Complex Molecular Systems, Macromolecular and Organic Chemistry, Self-Organizing Soft Matter
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Small Angle
Time Factors
Physiology
Lipid Bilayers
Cooperativity
Biochemistry
Phenylpropionates/chemistry
DSC
Scattering
0302 clinical medicine
Models
Reference Values
2-Naphthylamine
General Pharmacology
Toxicology and Pharmaceutics
Lipid bilayer
lcsh:QH301-705.5
2-Naphthylamine/analogs & derivatives
Artepillin C
lcsh:R5-920
Microscopy
Microscopy
Confocal
Phenylpropionates
Calorimetry
Differential Scanning
Chemistry
Small-angle X-ray scattering
General Neuroscience
Vesicle
Temperature
General Medicine
SAXS
METABOLISMO SECUNDÁRIO
Cholesterol
Membrane
030220 oncology & carcinogenesis
Confocal
Cholesterol/chemistry
Model membranes
Giant unilamellar vesicles
lcsh:Medicine (General)
Liposomes/chemistry
Lipid Bilayers/chemistry
Research Article
Immunology
Biophysics
Ocean Engineering
Chemical
Calorimetry
Differential Scanning
Thermotropic crystal
Fluorescence
03 medical and health sciences
Differential scanning calorimetry
Scattering
Small Angle
Reproducibility of Results
Biological membrane
Cell Biology
030104 developmental biology
lcsh:Biology (General)
Models
Chemical
Microscopy
Fluorescence
Liposomes
Green propolis
Laurates
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Brazilian Journal of Medical and Biological Research v.52 n.3 2019
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, 52(3):e8281. Scielo
Brazilian Journal of Medical and Biological Research, Vol 52, Iss 3 (2019)
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Brazilian Journal of Medical and Biological Research, Volume: 52, Issue: 3, Article number: e8281, Published: 25 MAR 2019
ISSN: 0100-879X
Popis: Made available in DSpace on 2022-04-29T08:45:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C, on model membranes (small and giant unilamelar vesicles) composed of ternary lipid mixtures containing cholesterol, which display liquid-ordered (lo) and liquid-disordered (ld) phase coexistence. Specifically, we explored potential changes in relevant membrane parameters upon addition of artepillin C present-ing both neutral and deprotonated states by means of small angle X-ray scattering (SAXS), differential scanning calorimetry (DSC), and confocal and multiphoton excitation fluorescence microscopy. Thermotropic analysis obtained from DSC experiments indicated a loss in the lipid cooperativity of lo phase at equilibrium conditions, while at similar conditions spontaneous formation of unilamellar vesicles from SAXS experiments showed that deprotonated artepillin C preferentially located at the surface of the membrane. Time-resolved experiments using fluorescence microscopy showed that at doses above 100 mM, artepillin C in its neutral state interacted with both liquid-ordered and liquid-disordered phases, inducing curvature stress and promoting dehydration at the membrane interface. Departmento de Física Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo Departmento de Física Faculdade de Ciências e Tecnologia Universidade do Estado de São Paulo Macromolecular and Organic Chemistry Physical Chemistry & Institute for Complex Molecular Systems Eindhoven University of Technology Dutch Polymer Institute (DPI) Departamento de Genética Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo CAPES: 004137/2014-00 FAPESP: 2014/26895 FAPESP: 2016/09633-4 CNPq: 232302/ 2014-6 CNPq: 304981/2012-5
Databáze: OpenAIRE
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