Human retinal pigment epithelial cells inhibit proliferation and IL2R expression of activated T cells
| Autor: | C G Kaestel, Mette Olaf Nielsen, Mogens Holst Nissen, Karsten W. Eriksen, Niels Ødum, A. Jørgensen, Carsten Röpke |
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| Rok vydání: | 2002 |
| Předmět: |
Male
T cell T-Lymphocytes Apoptosis Cell Communication Lymphocyte Activation Cell Line Cellular and Molecular Neuroscience medicine Cytotoxic T cell Humans IL-2 receptor Antigen-presenting cell Pigment Epithelium of Eye Interleukin 3 CD40 biology Cell Cycle Receptors Interleukin-2 Sensory Systems Coculture Techniques Cell biology Ophthalmology medicine.anatomical_structure Cell culture biology.protein Interleukin 12 Cell Division |
| Zdroj: | Experimental eye research. 74(5) |
| ISSN: | 0014-4835 |
| Popis: | The purpose of this study was to characterize the effects of human retinal pigment epithelial (RPE) cells on activated T cells. Activated T cells were cocultured with adult and foetal human RPE cells whereafter apoptosis and proliferation were determined by flow cytometry and (3)H-Thymidine incorporation assay, respectively. T cells and RPE cells were cultured directly together or in a transwell system for determination of the effect of cell contact. The importance of cell surface molecules was examined by application of a panel of blocking antibodies (CD2, CD18, CD40, CD40L, CD54, CD58) in addition to use of TCR negative T cell lines. The expression of IL2R-alpha -beta and -gamma chains of activated T cells was analysed by flow cytometry after incubation of T cells alone or with RPE cells. Human RPE cells were found to inhibit the proliferation of activated T cells by a cell contact-dependent mechanism. The RPE cells inhibitory abilities were not affected by blocking of any of the tested surface molecules. The inhibition of the T cells' proliferation correlates with a decreased expression of IL2R-beta and -gamma chains. The T cells regain their ability to proliferate and increase their IL2R-beta and -gamma chain expression within 24 hr after removal from the coculture. It is concluded that the cultured human adult and foetal RPE cells inhibit the proliferation of activated T cells by a process that does not involve apoptosis. It depends on cell contact but the involved surface molecules were not revealed. The proliferation inhibition correlates with a modulation of the T cells' expression of IL2R, and is reversible. |
| Databáze: | OpenAIRE |
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