Noscapine alleviates cerebral damage in ischemia-reperfusion injury in rats
| Autor: | Chandan Sahi, Avinash Mandloi, Vidhu Saxena, Vipin Dhote, Manisha Kawadkar, Chetana Tamadaddi |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Noscapine Saccharomyces cerevisiae Pharmacology medicine.disease_cause Neuroprotection Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Viability assay Muscle Strength Rats Wistar Cell damage Gait Peroxidase Chemistry Brain Infarction Middle Cerebral Artery General Medicine Glutathione medicine.disease Oxidative Stress 030104 developmental biology Neuroprotective Agents Reperfusion Injury Reperfusion injury 030217 neurology & neurosurgery Oxidative stress Psychomotor Performance medicine.drug |
| Zdroj: | Naunyn-Schmiedeberg's archives of pharmacology. 394(4) |
| ISSN: | 1432-1912 |
| Popis: | With high unmet medical needs, stroke remains an intensely focused research area. Although noscapine is a neuroprotective agent, its mechanism of action in ischemic-reperfusion (I-R) injury is yet to be ascertained. We investigated the effect of noscapine on the molecular mechanisms of cell damage using yeast, and its neuroprotection on cerebral I-R injury in rats. Yeast, both wild-type and Δtrx2 strains, was evaluated for cell growth and viability, and oxidative stress to assess the noscapine effect at 8, 10, 12, and 20 μg/ml concentrations. The neuroprotective activity of noscapine (5 and 10 mg/kg; po for 8 days) was investigated in rats using middle cerebral artery occlusion-induced I-R injury. Infarct volume, neurological deficit, oxidative stress, myeloperoxidase activity, and histological alterations were determined in I-R rats. In vitro yeast assays exhibited significant antioxidant activity and enhanced cell tolerance against oxidative stress after noscapine treatment. Similarly, noscapine pretreatment significantly reduced infarct volume and edema in the brain. The neurological deficit was decreased and oxidative stress biomarkers, superoxide dismutase activity and glutathione levels, were significantly increased while lipid peroxidation showed significant decrease in comparison to vehicle-treated I-R rats. Myeloperoxidase activity, an indicator of inflammation, was also reduced significantly in treated rats; histological changes were attenuated with noscapine. The study demonstrates the protective effect of noscapine in yeast and I-R rats by improving cell viability and attenuating neuronal damage, respectively. This protective activity of noscapine could be attributed to potent free radical scavenging and inhibition of inflammation in cerebral ischemia-reperfusion injury. |
| Databáze: | OpenAIRE |
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