Polymer Brush–GaAs Interface and Its Use as an Antibody-Compatible Platform for Biosensing
| Autor: | Maria C. DeRosa, Walid M. Hassen, Juliana Chawich, Jan J. Dubowski, Khalid Moumanis, Daniela T Marquez |
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| Přispěvatelé: | Laboratoire Nanotechnologies et Nanosystèmes [Sherbrooke] (LN2), Université de Sherbrooke (UdeS)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] (3IT), Université de Sherbrooke (UdeS) |
| Rok vydání: | 2021 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Materials science [SDV]Life Sciences [q-bio] General Chemical Engineering Interface (computing) Nanotechnology 02 engineering and technology 010402 general chemistry Polymer brush 01 natural sciences Article QD1-999 ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Atom-transfer radical-polymerization Biomolecule nutritional and metabolic diseases General Chemistry Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences Chemistry chemistry Compatibility (mechanics) Surface modification 0210 nano-technology Biosensor |
| Zdroj: | ACS Omega ACS Omega, ACS Publications, 2021, ⟨10.1021/acsomega.0c04954⟩ ACS Omega, Vol 6, Iss 11, Pp 7286-7295 (2021) |
| ISSN: | 2470-1343 |
| DOI: | 10.1021/acsomega.0c04954 |
| Popis: | Despite evidence showing that polymer brushes (PBs) are a powerful tool used in biosensing for minimizing nonspecific interactions, allowing for optimization of biosensing performance, and the fact that GaAs semiconductors have proven to have a remarkable potential for sensitive biomolecule detection, the combination of these two robust components has never been considered nor evaluated as a platform for biosensing applications. This work reports different methodologies to prepare and tune PBs on the GaAs interface (PB–GaAs) and their potential as useful platforms for antibody grafting, with the ultimate goal of demonstrating the innovative and attractive character of the PB–GaAs interfaces in the enhanced capture of antibodies and control of nonspecific interactions. Three different functionalization approaches were explored, one “grafting-to” and two “grafting-from,” in which atom transfer radical polymerization (ATRP) was performed, followed by their corresponding characterizations. Demonstration of the compatibility of Escherichia coli (E. coli) and Legionella pneumophila (Lp) antibodies with the PB–GaAs platform compared to the results obtained with conventional biosensing architectures developed for GaAs indicates the attractive potential for operation of a sensitive biosensor. Furthermore, these results showed that by carefully choosing the nature and preparation methodology of a PB–GaAs interface, it is possible to effectively tune the affinity of PB–GaAs-based sensors toward E. coli and Lp antibodies ultimately demonstrating the superior specificity of the developed biosensing platform. |
| Databáze: | OpenAIRE |
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