Cell suspension cultures of allogenic keratinocytes are efficient carriers for ex vivo gene transfer and accelerate the healing of full-thickness skin wounds by overexpression of human epidermal growth factor

Autor:	Elof Eriksson, Patrik Velander, Jan Vranckx, Akira Takedo, Christoph Theopold, Nicola Petrie, Daniela Hoeller, Feng Yao
Rok vydání:	2007
Předmět:	Keratinocytes medicine.medical_specialty Swine medicine.medical_treatment Genetic Vectors Cell Wound Breakdown Gene transfer Dermatology Epithelium Andrology Suspensions Animals Humans Transplantation Homologous Medicine Cells Cultured Wound Healing Epidermal Growth Factor integumentary system business.industry Human epidermal growth factor Growth factor Gene Transfer Techniques Transfection Surgery Transplantation medicine.anatomical_structure Models Animal Female business Ex vivo
Zdroj:	Wound Repair and Regeneration. 15:657-664
ISSN:	1524-475X 1067-1927
Popis:	The concept of using growth factor therapy to induce wound repair has been endorsed in studies that show reduced growth factors in wound fluid from chronic and aged wounds. In this study, we used cell suspensions of allogenic keratinocytes as gene-delivery vehicles for human epidermal growth factor (hEGF) and analyzed their impact on wound repair in a porcine wound-healing model. Full-thickness wounds were created on the backs of six Yorkshire pigs and covered with a wound chamber to create a wet wound-healing environment. First, 5 x 10(5) allogenic, autogenic, or mixed keratinocytes were transplanted into wounds and healing parameters were analyzed. Second, we measured long-term reepithelialization and contraction rates from day 8 until day 35. In the third experiment, allogenic keratinocytes were transfected with an hEGF-expressing plasmid pCEP-hEGF and transplanted in full-thickness wounds to improve repair. Wounds treated with autogenic, allogenic, or mixed keratinocytes showed a significantly higher rate of reepithelialization relative to saline-treated control wounds. Repetitive biopsies indicated that the use of allogenic keratinocytes did not lead to long-term wound breakdown. Wounds treated with hEGF-expressing allogenic keratinocytes reepithelialized faster than wounds treated with allogenic keratinocytes or control wounds. With a peak hEGF expression of 920.8 pg/mL, hEGF was detectable until day 5 after transplantation compared with minimal hEGF expression in control wounds. This study shows that allogenic keratinocytes can serve as efficient gene transfer vehicles for ex vivo growth factor delivery to full-thickness wounds and overexpression of hEGF further improves reepithelialization rates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea69c15787cb2af70ae41432b09080fa https://doi.org/10.1111/j.1524-475x.2007.00272.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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