Ethanol exposure leads to disorder of blood island formation in early chick embryo
| Autor: | Guang Wang, Bin-zhen Chen, Lin-rui Gao, Chaojie Wang, Xuesong Yang, Manli Chuai, Yongping Bao, Jing Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ethanol Hemangioblasts Cellular differentiation Embryogenesis Embryonic Development Cell Differentiation Cell migration Embryo Chick Embryo Biology Toxicology Embryonic stem cell Cell biology 03 medical and health sciences 030104 developmental biology Vasculogenesis Cell Movement embryonic structures Immunology Animals Blood islands Progenitor cell Reactive Oxygen Species |
| Zdroj: | Reproductive Toxicology. 73:96-104 |
| ISSN: | 0890-6238 |
| Popis: | Ethanol’s effect on embryonic vasculogenesis and its underlying mechanism is obscure. Using VE-cadherin in situ hybridization, we found blood islands formation was inhibited in area opaca, but abnormal VE-cadherin+ cells were seen in area pellucida. We hypothesise ethanol may affect blood island progenitor cell migration and differentiation. DiI and in vitro experiments revealed ethanol inhibited cell migration, Quantitative PCR analysis revealed that ethanol exposure enhanced cell differentiation in area pellucida of HH5 chick embryos and repressed cell differentiation in area pellucida of HH8 chick embryos. By exposing to 2,2′-azobis-amidinopropane dihydrochloride, a ROS inducer, which gave a similar anti-vasculogenesis effect as ethanol and this anti-vasculogenesis effect could be reversed by vitamin C. Overall, exposing early chick embryos to ethanol represses blood island progenitor cell migration but disturbed differentiation at a different stage, so that the disorder of blood island formation occurs through excess ROS production and altered vascular-associated gene expression. |
| Databáze: | OpenAIRE |
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