Synthesis of biopterin from dihydroneopterin triphosphate by rat tissues
| Autor: | Shin'ichi Yoshioka, Masahiro Masada, Takashi Mizokami, Kayo Inoue, Takeo Yoshida, Miki Akino |
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| Rok vydání: | 1983 |
| Předmět: |
Sepiapterin
Biophysics Biopterin Kidney Neopterin Pineal Gland Biochemistry High-performance liquid chromatography chemistry.chemical_compound Pineal gland medicine Animals Pterin Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification Pteridines Kidney metabolism Rats Inbred Strains Rats medicine.anatomical_structure Enzyme Liver chemistry Oxidation-Reduction Iodine |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 756:279-285 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/0304-4165(83)90336-7 |
| Popis: | High performance liquid chromatography procedure for the analysis of pterins of biopterin synthesis from dihydroneopterin triphosphate via sepiapterin in rat tissues has been described. Sepiapterin-synthesizing enzyme 1, which catalyzes in the presence of Mg2+ the conversion of dihydroneopterin triphosphate to an intermediate designated compound X was assayed by determining pterin which is formed from compound X under acidic conditions. Sepiapterin- and biopterin-synthesizing activity were also assayed by determining sepiapterin and biopterin, respectively. Analytical results revealed the presence of these activities in most rat tissues examined and high levels were found in kidney, pineal gland and liver. Activities were also detectable in peripheral erythrocytes. |
| Databáze: | OpenAIRE |
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