Cell dynamics in the correct control of bone metabolism using natural treatments
| Autor: | S. Amati, O. Gorrieri, Guendalina Lucarini, G. Suffritti, K. Kyriakidou, Monica Mattioli-Belmonte, Graziella Biagini, Milena Fini, Gianluca Giavaresi, Roberto Giardino |
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| Rok vydání: | 2006 |
| Předmět: |
Silicon
Time Factors Phalloidin Cell Survival Cell 030232 urology & nephrology Biomedical Engineering Medicine (miscellaneous) chemistry.chemical_element Bioengineering Video microscopy 030204 cardiovascular system & hematology Biology Calcium In Vitro Techniques Cell Line Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Cytoskeleton Cells Cultured Cell Proliferation Analysis of Variance Microscopy Video Osteoblasts General Medicine In vitro Staining medicine.anatomical_structure chemistry Biochemistry Microscopy Fluorescence Microscopy Electron Scanning Alkaline phosphatase |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0391-3988 |
| Popis: | The present study was undertaken in order to assess the efficacy of a commercial product containing calcium and silicon (Osteosil-Calcium®) on cell metabolism. MG-63 osteblast-like cells were cultured in the presence of three different drug concentrations (10, 5 and 2.5 μg/mL). Either serum-free culture and standard culture with serum were investigated. Morpho-functional tests (MTT and ALP), scanning electron microscopy (SEM), microanalysis (EDAX) and time-lapse video microscopy were performed. Cell actin cytoskeletal modification with fluorescence phalloidin staining was also tested. Our data show the in vitro functional efficacy of Osteosil-Calcium® on MG63 cell viability and ALP production. This study demonstrates its positive effect on the metabolism of the single cell and suggests wider uses of this drug in health protection and or in Regenerative Medicine therapies which are currently applied to the elderly |
| Databáze: | OpenAIRE |
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