PEGylated Terbium-Based Nanorods as Multimodal Bioimaging Contrast Agents
| Autor: | Ana M. Beltrán, Carlos Caro, Manuel Leal, María L. García-Martín, Jose María Páez-Muñoz |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | RIUMA. Repositorio Institucional de la Universidad de Málaga instname |
| ISSN: | 2574-0970 |
| DOI: | 10.1021/acsanm.1c00569 |
| Popis: | Diagnostic imaging strongly relies on the use of contrast agents (CAs). In general terms, current CAs present undesirable side effects that encourage researchers and pharmaceutical companies to continually search for safer and more versatile alternatives. Here, we describe the synthesis and characterization of terbium-based nanorods (TbNRs) as a potential alternative to traditional CAs for magnetic resonance imaging (MRI) and X-ray computed tomography (CT). The paramagnetism and high atomic number of Tb provide TbNRs with both magnetic relaxivity and X-ray attenuation capabilities. After surface functionalization with a polyethylene glycol (PEG)-derived ligand, TbNRs showed high colloidal stability in physiological media. Additionally, toxicity studies conducted in cell cultures and zebrafish embryos demonstrated the safety of the as-synthesized TbNRs, thus supporting their potential use as CAs. Lastly, in vivo imaging experiments in mice demonstrated that TbNRs produce remarkable contrast enhancement on both MRI and CT. The authors want to express special thanks to Alejandro Domínguez for his invaluable help with the in vivo toxicity experiments. The authors also thank Dr. Juan F. López for his support with the TEM experiments, Dr. Iñaki Orue for VSM measurement, Dr. John Pearson and Luisa Macías for assistance with the cell experiments and helpful discussion, Reyes Molina for assistance with animal experiments, and Maria Somoza for helping with the MRI experiments. Authors thank BIONAND’s Nanoimaging Unit. Optical microscopy, TEM, CT, and MRI experiments have been performed in the ICTS “NANBIOSIS”, more specifically in the U28 Unit at BIONAND. Funding for open access charge: Universidad de Málaga / CBUA. The authors want to express special thanks to Alejandro Domínguez for his invaluable help with the in vivo toxicity experiments. The authors also thank Dr. Juan F. López for his support with the TEM experiments, Dr. Iñaki Orue for VSM measurement, Dr. John Pearson and Luisa Macías for assistance with the cell experiments and helpful discussion, Reyes Molina for assistance with animal experiments, and Maria Somoza for helping with the MRI experiments. Authors thank BIONAND’s Nanoimaging Unit. Optical microscopy, TEM, CT, and MRI experiments have been performed in the ICTS “NANBIOSIS”, more specifically in the U28 Unit at BIONAND. |
| Databáze: | OpenAIRE |
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