Detection of Tumor-Specific Antigens in Philadelphia Chromosome Positive Leukemias
| Autor: | P. Ten Hacken, J van Denderen, W. Van Ewijk, P. Berendes |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cancer Research
Molecular Sequence Data Fusion Proteins bcr-abl Genes abl Biology Philadelphia chromosome Sensitivity and Specificity Epitope Epitopes Antigens Neoplasm Leukemia Myelogenous Chronic BCR-ABL Positive hemic and lymphatic diseases Biomarkers Tumor medicine Humans Philadelphia Chromosome Amino Acid Sequence neoplasms ABL Philadelphia Chromosome Positive breakpoint cluster region Myeloid leukemia Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Fusion protein Molecular biology Peptide Fragments Leukemia Oncology |
| Zdroj: | Leukemia & Lymphoma. 11:29-32 |
| ISSN: | 1029-2403 1042-8194 |
| DOI: | 10.3109/10428199309047859 |
| Popis: | In chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL) the Ph1 chromosome (22q-) is the most frequent chromosomal aberration encountered. At the molecular level the c-abl gene from chr. 9 is translocated to the bcr gene on chr. 22. As a result, a chimeric bcr-abl gene is generated, which encodes chimeric proteins. Since these proteins are only expressed in Ph1 positive cells, they are per definition tumor-specific. In this report we describe the reactivity of polyvalent antisera raised against synthetic peptides corresponding to the tumor-specific bcr-abl junctions. Native chimeric proteins were specifically recognized by these junction-specific antisera. Therefore we conclude that the bcr-abl junctions are antigenically exposed on the chimeric proteins. We discuss the relevance of these antisera for CML and ALL diagnosis. |
| Databáze: | OpenAIRE |
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