Cell Surface Expression of Nrg1 Protein in Candida auris
| Autor: | Govindsamy Vediyappan, Anuja Paudyal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Hyphal growth Hypha drug-resistant Plant Science Article sweat medium Microbiology 03 medical and health sciences Western blot anti-Nrg1 antibody medicine cell surface proteins immunofluorescence Zinc ion binding lcsh:QH301-705.5 Ecology Evolution Behavior and Systematics 030304 developmental biology BME extract Gel electrophoresis 0303 health sciences cell surface-associated Nrg1 medicine.diagnostic_test 030306 microbiology Chemistry Biofilm 2-DE Corpus albicans Candida auris lcsh:Biology (General) Zn-finger protein Candida auris biofilm |
| Zdroj: | Journal of Fungi Volume 7 Issue 4 Journal of Fungi, Vol 7, Iss 262, p 262 (2021) |
| ISSN: | 2309-608X |
| Popis: | Candida auris is an emerging antifungal resistant human fungal pathogen increasingly reported in healthcare facilities. It persists in hospital environments, and on skin surfaces, and can form biofilms readily. Here, we investigated the cell surface proteins from C. auris biofilms grown in a synthetic sweat medium mimicking human skin conditions. Cell surface proteins from both biofilm and planktonic control cells were extracted with a buffer containing β-mercaptoethanol and resolved by 2-D gel electrophoresis. Some of the differentially expressed proteins were excised and identified by mass spectrometry. C. albicans orthologs Spe3p, Tdh3p, Sod2p, Ywp1p, and Mdh1p were overexpressed in biofilm cells when compared to the planktonic cells of C. auris. Interestingly, several proteins with zinc ion binding activity were detected. Nrg1p is a zinc-binding transcription factor that negatively regulates hyphal growth in C. albicans. C. auris does not produce true hypha under standard in vitro growth conditions, and the role of Nrg1p in C. auris is currently unknown. Western blot analyses of cell surface and cytosolic proteins of C. auris against anti-CalNrg1 antibody revealed the Nrg1p in both locations. Cell surface localization of Nrg1p in C. auris, an unexpected finding, was further confirmed by immunofluorescence microscopy. Nrg1p expression is uniform across all four clades of C. auris and is dependent on growth conditions. Taken together, the data indicate that C. auris produces several unique proteins during its biofilm growth, which may assist in the skin-colonizing lifestyle of the fungus during its pathogenesis. |
| Databáze: | OpenAIRE |
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