Iron oxide nanoparticles based antiviral activity of H1N1 influenza A virus
| Autor: | Muktikant Nayak, Major Madhukar, Krishna Pandey, V. N. R. Das, Rishikesh Kumar, Roshan Kamal Topno, Ganesh Chandra Sahoo, Pradeep Das, Mamta Chawla Sarkar, Yousuf Ansari, Bhawna |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Cell Survival viruses 030106 microbiology Iron oxide Metal Nanoparticles Peptide Microbial Sensitivity Tests Antiviral Agents Ferric Compounds Virus Cell Line Inhibitory Concentration 50 03 medical and health sciences chemistry.chemical_compound Influenza A Virus H1N1 Subtype 0302 clinical medicine Influenza Human Animals Humans Pharmacology (medical) MTT assay 030212 general & internal medicine Viability assay IC50 chemistry.chemical_classification virus diseases Haplorhini Virology Infectious Diseases Real-time polymerase chain reaction chemistry Iron oxide nanoparticles |
| Zdroj: | Journal of Infection and Chemotherapy. 25:325-329 |
| ISSN: | 1341-321X |
| Popis: | Influenza virus is a common human pathogenic agent that has caused serious respiratory illness and death over the past century and in recent year. Treatment options against pandemic influenza strain A/H1N1 are very limited and unsatisfactory. Therefore we have developed iron oxide nanoparticles (IO-NPs) with particle size in the range of 10–15 nm against pandemic influenza strain A/H1N1/Eastern India/66/PR8-H1N1. Cell viability and anti-influenza activity was measured by MTT assay, plaque inhibition and quantifying viral transcripts using quantitative real-time PCR with Iron oxide nanoparticles in a dose- and time-dependent manner. 50% cell viability (TD50) was observed at 4.25 pg ± .2 pg of Iron oxide nanoparticles. The percentage of plaque inhibition relative to the infection and the IC50 (50% virus reduction) of PR8-H1N1strain (0.5 moi) were measured in vitro by the plate forming unit (pfu) in MA104 cells. Finding were observed at 01 pg after 72 h. The Antiviral activity determined by change in viral RNA transcripts within 24 h of virus infection by RT-PCR, 08 fold reductions in virus found when treated with Iron oxide nanoparticles Thus; it opens a new avenue for use of IP-NPs against virus infections. |
| Databáze: | OpenAIRE |
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