Ventricular tachyarrhythmias in rats with acute myocardial infarction involves activation of small-conductance Ca2+-activated K+ channels

Autor: Zixi Jack Cheng, Yin-Yu Jia, Jianhua Zhu, Le Gui, Zhi-Wei Bao, Xiaotong Qin, Qing-Hui Chen
Rok vydání: 2013
Předmět:
Male
Tachycardia
medicine.medical_specialty
Time Factors
Refractory Period
Electrophysiological
Small-Conductance Calcium-Activated Potassium Channels
Physiology
Refractory period
Myocardial Infarction
Action Potentials
Rats
Sprague-Dawley
Electrocardiography
Heart Rate
Physiology (medical)
Internal medicine
Alkanes
Potassium Channel Blockers
Animals
Medicine
Myocyte
cardiovascular diseases
Myocardial infarction
Dose-Response Relationship
Drug
medicine.diagnostic_test
business.industry
Quinolinium Compounds
Cardiac Pacing
Artificial
medicine.disease
Rats
Disease Models
Animal
Electrophysiology
Apamin
Ventricular Fibrillation
Ventricular fibrillation
Tachycardia
Ventricular
Cardiology
Myocardial infarction complications
medicine.symptom
Cardiology and Cardiovascular Medicine
business
Anti-Arrhythmia Agents
Zdroj: American Journal of Physiology-Heart and Circulatory Physiology. 304:H118-H130
ISSN: 1522-1539
0363-6135
DOI: 10.1152/ajpheart.00820.2011
Popis: In vitro experiments have shown that the upregulation of small-conductance Ca2+-activated K+ (SK) channels in ventricular epicardial myocytes is responsible for spontaneous ventricular fibrillation (VF) in failing ventricles. However, the role of SK channels in regulating VF has not yet been described in in vivo acute myocardial infarction (AMI) animals. The present study determined the role of SK channels in regulating spontaneous sustained ventricular tachycardia (SVT) and VF, the inducibility of ventricular tachyarrhythmias, and the effect of inhibition of SK channels on spontaneous SVT/VF and electrical ventricular instability in AMI rats. AMI was induced by ligation of the left anterior descending coronary artery in anesthetized rats. Spontaneous SVT/VF was analyzed, and programmed electrical stimulation was performed to evaluate the inducibility of ventricular tachyarrhythmias, ventricular effective refractory period (VERP), and VF threshold (VFT). In AMI, the duration and episodes of spontaneous SVT/VF were increased, and the inducibility of ventricular tachyarrhythmias was elevated. Pretreatment in the AMI group with the SK channel blocker apamin or UCL-1684 significantly reduced SVT/VF and inducibility of ventricular tachyarrhythmias ( P < 0.05). Various doses of apamin (7.5, 22.5, 37.5, and 75.0 μg/kg iv) inhibited SVT/VF and the inducibility of ventricular tachyarrhythmias in a dose-dependent manner. Notably, no effects were observed in sham-operated controls. Additionally, VERP was shortened in AMI animals. Pretreatment in AMI animals with the SK channel blocker significantly prolonged VERP ( P < 0.05). No effects were observed in sham-operated controls. Furthermore, VFT was reduced in AMI animals, and block of SK channels increased VFT in AMI animals, but, again, this was without effect in sham-operated controls. Finally, the monophasic action potential duration at 90% repolarization (MAPD90) was examined in the myocardial infarcted (MI) and nonmyocardial infarcted areas (NMI) of the left ventricular epicardium. Electrophysiology recordings showed that MAPD90 in the MI area was shortened in AMI animals, and pretreatment with SK channel blocker apamin or UCL-1684 significantly prolonged MAPD90 ( P < 0.05) in the MI area but was without effect in the NMI area or in sham-operated controls. We conclude that the activation of SK channels may underlie the mechanisms of spontaneous SVT/VF and suseptibility to ventricular tachyarrhythmias in AMI. Inhibition of SK channels normalized the shortening of MAPD90 in the MI area, which may contribute to the inhibitory effect on spontaneous SVT/VF and inducibility of ventricular tachyarrhythmias in AMI.
Databáze: OpenAIRE
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