Fundamental biomaterial properties of tough glycosaminoglycan-containing double network hydrogels newly developed using the molecular stent method

Autor: Nobuto Kitamura, Takayuki Nonoyama, Kazuyuki Sugahara, Kotaro Higa, Susumu Wada, Jian Ping Gong, Keiko Goto, Kazunori Yasuda, Fuminori Kanaya, Takayuki Kurokawa
Rok vydání: 2016
Předmět:
Materials science
Biomedical Engineering
Biocompatible Materials
02 engineering and technology
Buffers
010402 general chemistry
01 natural sciences
Biochemistry
Biomaterials
Glycosaminoglycan
chemistry.chemical_compound
Subcutaneous Tissue
Implants
Experimental
Materials Testing
medicine
Animals
Humans
Chondroitin
Chondroitin sulfate
Hyaluronic Acid
Collagen Type II
Molecular Biology
Cells
Cultured
Glycosaminoglycans
Mechanical Phenomena
Inflammation
Muscles
Cartilage
Regeneration (biology)
Chondroitin Sulfates
Sterilization
Water
Biomaterial
Cell Differentiation
Hydrogels
General Medicine
021001 nanoscience & nanotechnology
Chondrogenesis
0104 chemical sciences
medicine.anatomical_structure
chemistry
Self-healing hydrogels
Female
Rabbits
0210 nano-technology
Biotechnology
Biomedical engineering
Zdroj: Acta Biomaterialia. 43:38-49
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2016.07.023
Popis: The purpose of this study was to clarify fundamental mechanical properties and biological responses of the sodium hyaluronate-containing double network (HA-DN) gel and chondroitin sulfate-containing double network (CS-DN) gel, which were newly developed using the molecular stent method. This study discovered the following facts. First, these hydrogels had high mechanical performance comparable to the native cartilage tissue, and the mechanical properties were not affected by immersion in the saline solution for 12 weeks. Secondly, the mechanical properties of the CS-DN gel were not significantly reduced at 12 weeks in vivo, while the mechanical properties of the HA-DN gel were significantly deteriorated at 6 weeks. Thirdly, the degree of inflammation around the HA-DN gel was the same as that around the negative control. The CS-DN gel showed a mild but significant foreign body reaction, which was significantly greater than the negative control and less than the positive control at 1 week, while the inflammation was reduced to the same level as the negative control at 4 and 6 weeks. Fourthly, these gels induced differentiation of the ATDC5 cells into chondrocytes in the culture with the insulin-free maintenance medium. These findings suggest that these tough hydrogels are potential biomaterials for future application to therapeutic implants such as artificial cartilage. Statement of Significance The present study reported fundamental biomaterial properties of the sodium hyaluronate-containing double network (HA-DN) gel and chondroitin sulfate-containing double network (CS-DN) gel, which were newly developed using the molecular stent method. Both the HA- and CS-DN gels had high mechanical properties comparable to the cartilage tissue and showed the ability to induce chondrogenic differentiation of ATDC5 cells in vitro. They are potential biomaterials that may meet the requirements of artificial cartilage concerning the material properties. Further, these DN gels can be also applied to the implantable inducer for cell-free cartilage regeneration therapy.
Databáze: OpenAIRE
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