Synthesis and evaluation of L-cystathionine as a standard for amino acid analysis
| Autor: | Yusuke Amino, Yumiko Suzuki |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Clinical Chemistry Tests
Chemistry Techniques Synthetic 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Amino acid analysis Cystathionine Molecular Biology chemistry.chemical_classification Chromatography biology 010405 organic chemistry Chemistry Organic Chemistry General Medicine Reference Standards Cystathionine beta synthase Quantitative determination 0104 chemical sciences Amino acid Certified reference materials Clinical diagnosis biology.protein Biotechnology |
| DOI: | 10.6084/m9.figshare.3814248.v1 |
| Popis: | L-Cystathionine is a key nonprotein amino acid related to metabolic conditions. The quantitative determination of L-cystathionine in physiological fluids by amino acid analysis is important for clinical diagnosis; however, certified reference material for L-cystathionine with satisfactory purity, content, and quantity has been unavailable until recently. Consequently, a practical and simple method for the preparation of L-cystathionine was examined, which involves thioalkylation of N-tert-butoxycarbonyl-L-cysteine tert-butyl ester, derived from L-cystine, with (2S)-2-(tert-butoxycarbonyl)amino-4-iodobutanoic acid tert-butyl ester, derived from L-aspartic acid, to obtain L-cystathionine with protecting groups, followed by single-step deprotection under mild conditions. This method produces L-cystathionine in high purity (99.4%) and having sufficient percentage content according to amino acid analysis, which could be used as a standard for the amino acid analysis of physiological fluids. Synthesis and amino acid analysis chromatogram of L-cystathionine. |
| Databáze: | OpenAIRE |
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