Increasing the Efficacy of Stem Cell Therapy via Triple-Function Inorganic Nanoparticles
| Autor: | Natalia I. Gonzalez-Pech, David J. Cheng, Ghanim Hableel, George L. Sen, Jesse V. Jokerst, Yijun Xie, Fang Chen, Tao Hu, Vicki H. Grassian, Taeho Kim, Eric Zhao, Jingting Li, Jeanne E. Lemaster |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_treatment
Cell Myocardial Infarction General Physics and Astronomy Nanoparticle Contrast Media 02 engineering and technology 010402 general chemistry Mesenchymal Stem Cell Transplantation 01 natural sciences Ferric Compounds Theranostic Nanomedicine Article Mice medicine Animals Humans General Materials Science Viability assay Insulin-Like Growth Factor I Cells Cultured Chemistry General Engineering Mesenchymal Stem Cells Stem-cell therapy 021001 nanoscience & nanotechnology Silicon Dioxide Magnetic Resonance Imaging 0104 chemical sciences Mice Inbred C57BL Drug Liberation medicine.anatomical_structure Drug delivery Biophysics Nanomedicine Nanoparticles Stem cell 0210 nano-technology Superparamagnetism |
| Zdroj: | ACS Nano |
| ISSN: | 1936-086X |
| Popis: | Stem cell therapy in heart disease is challenged by mis-injection, poor survival, and low cell retention. Here, we describe a biocompatible multifunctional silica–iron oxide nanoparticle to help solve these issues. The nanoparticles were made via an in situ growth of Fe(3)O(4) nanoparticles on both the external surfaces and pore walls of mesocellular foam silica nanoparticles. In contrast to previous work, this approach builds a magnetic moiety inside the pores of a porous silica structure. These materials serve three roles: drug delivery, magnetic manipulation, and imaging. The addition of Fe(3)O(4) to the silica nanoparticles increased their colloidal stability, T(2)-based magnetic resonance imaging contrast, and superparamagnetism. We then used the hybrid materials as a sustained release vehicle of insulin-like growth factor—a pro-survival agent that can increase cell viability. In vivo rodent studies show that labeling stem cells with this nanoparticle increased the efficacy of stem cell therapy in a ligation/reperfusion model. The nanoparticle-labeled cells increase the mean left ventricular ejection fraction by 11 and 21% and the global longitudinal strain by 24 and 34% on days 30 and 60, respectively. In summary, this multifunctional nanomedicine improves stem cell survival via the sustained release of pro-survival agents. |
| Databáze: | OpenAIRE |
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